Bergstrom R F, Kay D R, Wagner J G
J Chromatogr. 1981 Mar 13;222(3):445-52. doi: 10.1016/s0378-4347(00)84145-6.
A high-performance liquid chromatographic method for the determination of penicillamine in plasma, whole blood, and urine samples is described. The method uses a commercially available electrochemical detector at a potential of +0.1 V versus the Ag/AgCl reference electrode. This method is selective and sensitive for sulfhydryl compounds. The chromatography separates penicillamine from other endogenous sulfhydryl compounds with a limit of detection for penicillamine in biological samples of ca. 10(07) M.
本文描述了一种用于测定血浆、全血和尿液样本中青霉胺的高效液相色谱法。该方法使用市售的电化学检测器，相对于Ag/AgCl参比电极的电位为+0.1 V。此方法对巯基化合物具有选择性和灵敏性。该色谱法可将青霉胺与其他内源性巯基化合物分离，生物样本中青霉胺的检测限约为10⁻⁷ M。
