一种用于在马-达二氏犬肾（MDCK）细胞中检测流感病毒的简化蚀斑测定法。
A simplified plaque assay for influenza viruses in Madin-Darby kidney (MDCK) cells.
作者信息
Huprikar J, Rabinowitz S
出版信息
J Virol Methods. 1980;1(2):117-20. doi: 10.1016/0166-0934(80)90020-8.
PMID:7228969
Abstract
A variety of influenza A and B viruses plaque in MDCK cell in trypsin is added only at the time of viral adsorption to the monolayer. Therefore, a conventional soft-agar overlay can be employed without addition of proteolytic enzymes. Plaquing efficiency was comparable to that when embryonated eggs were used to determine infectivity. Finally the method is simple and economical.
摘要
多种甲型和乙型流感病毒在MDCK细胞中形成蚀斑，胰蛋白酶仅在病毒吸附到单层细胞时添加。因此，可以采用传统的软琼脂覆盖法，无需添加蛋白水解酶。蚀斑形成效率与使用鸡胚测定感染性时相当。最后，该方法简单且经济。
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