Vanderbroucke A C, Cairns J A, Missirlis E, Gauldie J
J Immunoassay. 1980;1(2):251-69. doi: 10.1080/01971528008055787.
A radioimmunoassay specific for dog myoglobin (MGB) has been developed. Antibody (Ab) to purified dog heart MGB was prepared in rabbits and the Ab was labelled with 125I using the chloramine-T procedure. The assay is conducted at room temperature with addition of second Ab at 24 hr, the forward reaction being 95% complete at this point and irreversible. The assay is free of matrix affects. The working range is 20 to 360 ng/ml. Coefficients of variation for within run replicates are 10.5% (low) and 4.4% (high), and for between run replicates are 13.8% (low) and 7.6% (high). Serial measurement of serum MGB in dogs undergoing experimental myocardial infarction indicates that appearance time, peak time, and disappearance rate are respectively, 1.1 hr, 7.7 hr and 0.0025 min-1 for MGB and 2.6 hr, 13.35 hr, and 0.0018 min-1 for CK.
已开发出一种针对犬肌红蛋白（MGB）的放射免疫测定法。用纯化的犬心脏MGB免疫家兔制备抗体（Ab），并采用氯胺-T法用125I标记该抗体。该测定在室温下进行，24小时时加入第二抗体，此时正向反应已完成95%且不可逆。该测定不受基质影响。工作范围为20至360 ng/ml。批内重复测定的变异系数分别为低浓度10.5%和高浓度4.4%，批间重复测定的变异系数分别为低浓度13.8%和高浓度7.6%。对实验性心肌梗死犬血清MGB的系列测定表明，MGB的出现时间、峰值时间和消失率分别为1.1小时、7.7小时和0.0025分钟-1，肌酸激酶（CK）的相应数值分别为2.6小时、13.35小时和0.0018分钟-1。
