Adams E C, Layman K M, Jaunakais I
Ann Clin Lab Sci. 1978 Jul-Aug;8(4):330-41.
A column flow-through radioimmune assay for serum myoglobin has been developed. The assay uses antibodies to human or rhesus monkey myoglobin coupled to Sepharose by cyanogen bromide activation and myoglobin labeled by the Chloramine-T method. The labeled myoglobin is stable over two to three half-lives. Serum myoglobin levels were elevated when serum was taken up to 12 hours after myocardial infarction and generally elevated up to 24 hours. A continued or repeated elevation of serum myoglobin probably indicates continued myocardial damage or new attacks. In the cases of myocardial infarction, levels up to 1340 ng per ml were found.
已开发出一种用于检测血清肌红蛋白的柱流式放射免疫分析法。该分析法使用通过溴化氰活化偶联到琼脂糖上的抗人或恒河猴肌红蛋白抗体，以及用氯胺 - T法标记的肌红蛋白。标记的肌红蛋白在两到三个半衰期内稳定。心肌梗死后12小时内采集的血清中肌红蛋白水平升高，一般在24小时内都保持升高。血清肌红蛋白持续或反复升高可能表明心肌持续损伤或有新的发作。在心肌梗死病例中，发现每毫升水平高达1340纳克。
