Terwilliger T C, Solomon A K
J Gen Physiol. 1981 May;77(5):549-70. doi: 10.1085/jgp.77.5.549.
The osmotic water permeability of human red cells has been reexamined with a stopped-flow device and a new perturbation technique. Small osmotic gradients are used to minimize the systematic error caused by nonlinearities in the relationship between cell volume and light scattering. Corrections are then made for residual systematic error. Our results show that the hydraulic conductivity, Lp, is essentially independent of the direction of water flow and of osmolality in the range 184-365 mosM. the mean value of Lp obtained obtained was 1.8 +/- 0.1 (SEM) X 10-11 cm3 dyne -1 s-1.
利用停流装置和一种新的扰动技术,对人红细胞的渗透水通透性进行了重新研究。使用小的渗透梯度,以尽量减少细胞体积与光散射之间非线性关系所导致的系统误差。然后对残余的系统误差进行校正。我们的结果表明,在184 - 365毫渗摩尔范围内,水力传导率Lp基本上与水流方向和渗透压无关。所获得的Lp平均值为1.8 +/- 0.1(标准误)×10-11立方厘米·达因-1·秒-1。