用放射免疫分析法检测乙型肝炎核心抗原的IgM类抗体：两种方法的比较。

Detection by radioimmunoassay of IgM class antibody to hepatitis B core antigen: a comparison of two methods.

作者信息

Tedder R S, Wilson-Croome R

出版信息

J Med Virol. 1980;6(3):235-47. doi: 10.1002/jmv.1890060307.

Abstract

A solid-phase radioimmunoassay (RIA) for IgM anti-HBc is described. The assay (anti-mu RIA) is based on the adsorption of IgM to a tube coated with sheep antibody to the human IgM mu-chain. The adsorbed immunoglobulin is assayed for anti-HBc activity. Positive reactions in the test are shown to be due to IgM antibody, and a confirmation blocking test is described. Reactivity of test sera can be quantitated by comparison with standard sera containing known levels of IgM anti-HBc. Sera assayed for IgM anti-HBc by both the anti-mu RIA and a method employing serum fractionation and competitive RIA gave similar results. Rheumatoid factor (RF) alone did not react in the anti-mu RIA, but reactivity could be generated in the presence of RF and preformed aggregates of IgG anti-HBc.

摘要

本文描述了一种用于检测IgM抗-HBc的固相放射免疫分析（RIA）方法。该分析方法（抗μ链RIA）基于IgM吸附到包被有抗人IgM μ链羊抗体的试管上。对吸附的免疫球蛋白进行抗-HBc活性检测。试验中的阳性反应表明是由IgM抗体引起的，并描述了一种确认阻断试验。通过与含有已知水平IgM抗-HBc的标准血清比较，可以对检测血清的反应性进行定量。采用抗μ链RIA以及血清分级分离和竞争性RIA方法检测IgM抗-HBc的血清，结果相似。单独的类风湿因子（RF）在抗μ链RIA中不发生反应，但在存在RF和预先形成的IgG抗-HBc聚集体时可产生反应性。