Bayne W F, East T, Dye D
J Pharm Sci. 1981 Apr;70(4):458-9. doi: 10.1002/jps.2600700431.
A rapid and sensitive method for the analysis of indomethacin in plasma and urine was developed using high-pressure liquid chromatography, postcolumn, in-line hydrolysis of indomethacin to a fluorophore, and detection of the fluorophore wih a fluorometer. The lower limit of detection was 1.5 ng/ml of plasma. The coefficient of variation at 30 ng/ml of plasma was 4.5% (n = 5). The nonconjugated metabolites of indomethacin, aspirin, and salicylate were resolved from indomethacin and the internal standard, alpha-methylindomethacin.
建立了一种快速灵敏的血浆和尿液中吲哚美辛分析方法，该方法采用高压液相色谱法，对吲哚美辛进行柱后在线水解生成荧光团，并用荧光计检测该荧光团。检测下限为血浆中1.5 ng/ml。血浆中30 ng/ml时的变异系数为4.5%（n = 5）。吲哚美辛、阿司匹林和水杨酸盐的非共轭代谢物与吲哚美辛和内标α-甲基吲哚美辛得以分离。
