用电子捕获气液色谱法测定血清和尿液中的吲哚美辛。

Determination of indomethacin in serum and urine by electron-capture gas-liquid chromatography.

作者信息

Helleberg I

出版信息

J Chromatogr. 1976 Feb 4;117(1):167-73. doi: 10.1016/s0021-9673(00)81078-x.

Abstract

A specific and sensitive method for the quantitative determination of indomethacin in serum and urine is described. The drug is extracted at pH 5.0 with 1,2-dichloroethane and a portion of the organic extract is concentrated and made to react with diazoethane in diethyl ether. The ethyl ester derivative is analyzed by electron-capture gas-liquid chromatography, quantitation being achieved by comparison of peak areas for samples and standards, which are prepared in serum or urine and treated in the same manner as the samples. The limit of sensitivity is 50 ng/ml and the relative standard derivation for repeat determinations on the same sample is about 3%.

摘要

本文描述了一种用于定量测定血清和尿液中吲哚美辛的特异性灵敏方法。该药物在pH 5.0条件下用1,2 - 二氯乙烷萃取，取一部分有机萃取液浓缩后使其与乙醚中的重氮乙烷反应。通过电子捕获气液色谱法分析乙酯衍生物，通过比较样品和标准品的峰面积进行定量，标准品在血清或尿液中制备，并与样品以相同方式处理。灵敏度极限为50 ng/ml，对同一样品进行重复测定的相对标准偏差约为3%。