Lutz H U
J Supramol Struct. 1978;8(3):375-89. doi: 10.1002/jss.400080314.
When human erythrocytes are depleted of endogenous ATP they release spectrin-free vesicles as a light vesicle fraction [Lutz et al: J Cell Biol 73: 548, 1977] and chains of rounded vesicles as well as flattened myelin forms in a heavy vesicle fraction. The heavy fraction retains some spectrin, and glycophorin is partially degraded. The release of both types of fragments is not dependent upon added Ca+2, and 50 micrometer EGTA does not prevent the vesicle release. Concomitant with vesicle release, a large fraction of the major protein components of the cell is found in disulfide-bonded aggregates. A protocol is outlined to recover erythrocyte-specific fragments from thrombocyte-rich plasma. It allows detection of spectrin-free vesicles in whole blood stored under blood bank conditions for 12 days. In freshly drawn blood no such vesicles are observed, but particles are obtained that are different from thrombocyte fragments and that show a prominent glycoprotein running slightly faster than glycophorin.
当人类红细胞内源性ATP耗尽时，它们会释放无血影蛋白的囊泡作为轻囊泡组分[Lutz等人：《细胞生物学杂志》73: 548, 1977]，并在重囊泡组分中释放圆形囊泡链以及扁平的髓鞘样结构。重组分保留了一些血影蛋白，而血型糖蛋白部分降解。两种类型片段的释放不依赖于添加的Ca+2，50微米的乙二醇双四乙酸（EGTA）并不能阻止囊泡释放。与囊泡释放同时发生的是，细胞的大部分主要蛋白质成分以二硫键结合的聚集体形式存在。概述了一种从富含血小板的血浆中回收红细胞特异性片段的方案。它能够检测在血库条件下储存12天的全血中的无血影蛋白囊泡。在新鲜采集的血液中未观察到此类囊泡，但获得的颗粒不同于血小板片段，且显示出一种比血型糖蛋白迁移速度稍快的突出糖蛋白。
