用激光微束对培养神经元生长锥的钙动作电位进行光学记录。
Optical recording of calcium action potentials from growth cones of cultured neurons with a laser microbeam.
作者信息
Grinvald A, Farber I C
出版信息
Science. 1981 Jun 5;212(4499):1164-7. doi: 10.1126/science.7233210.
PMID:7233210
Abstract
Simultaneous recordings of calcium action potentials directly from growth cones and from somata of neuroblastoma cells indicated that they could be generated in the neurites at or near growth cones. Growth cone responses were measured with a fluorescent voltage-sensitive dye and a 5-milliwatt helium-neon laser microbeam as a monitoring light source.
摘要
直接从神经母细胞瘤细胞的生长锥和胞体同时记录钙动作电位，结果表明它们可能在生长锥处或其附近的神经突中产生。使用荧光电压敏感染料和5毫瓦氦氖激光微束作为监测光源来测量生长锥反应。
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