Wagner T, Peschel U, Heydrich D, Ritter U
Z Gastroenterol. 1981 Mar;19(3):140-7.
The immunoreactivity of patients with colorectal or pancreatic cancer was investigated by a modified leukocyte adherence inhibition test (LAI). The microcapillary-LAI-test was easy to practise and measurements had a low intra-assay coefficient of variation. The number of false positive results was low, only 1 of 39 patients with benign disease and not one of 13 healthy volunteers reacted in the presence of colorectal carcinoma extracts. In 43 patients with colorectal cancer 17 of 25 (68%) with Dukes' B & C and 15 of 18 (83%) with Dukes' D cancer were LAI positive. Of the 19 patients with colon polyps 6 positive results were observed, exclusively in case of large villous adenomas, polyposis coli and one polyp with a focal carcinoma. 10 of 11 patients with pancreatic cancer reacted in the test. Leukocytes from patients with limited cancer of other source, when tested with colorectal or pancreatic carcinoma extracts, showed a negative LAI response. However, in case of metastatic diseases some crossreactions were observed, which resulted in false positive tests. Following our results, the LAI test could be of value in the diagnosis of gastrointestinal carcinoma.
采用改良白细胞黏附抑制试验（LAI）对结直肠癌或胰腺癌患者的免疫反应性进行了研究。微毛细管LAI试验易于操作，测量的批内变异系数较低。假阳性结果数量较少，39例良性疾病患者中只有1例，13名健康志愿者在结直肠癌提取物存在下均无反应。在43例结直肠癌患者中，25例Dukes B和C期患者中有17例（68%）、18例Dukes D期患者中有15例（83%）LAI呈阳性。19例结肠息肉患者中，有6例呈阳性结果，仅见于大绒毛腺瘤、结肠息肉病及1例伴有局灶性癌的息肉患者。11例胰腺癌患者中有10例在试验中呈反应阳性。其他来源局限性癌症患者的白细胞在用结直肠癌或胰腺癌提取物检测时，LAI反应呈阴性。然而，在转移性疾病情况下，观察到一些交叉反应，导致假阳性检测结果。根据我们的研究结果，LAI试验在胃肠道癌的诊断中可能具有一定价值。
