Fujii S, Okamura H, Takenaka A, Kanzaki H, Okuda Y, Morimoto K, Nishimura T, Nakashima N
Acta Obstet Gynaecol Jpn. 1981 Feb;33(2):193-200.
It has been reported that multiple peritoneal fibroids were produced in guinea pigs treated with large doses of estrogens, and the nodules regressed when the steroid stimulant was discontinued. Recently, these experimental nodules were considered to be simulating the disseminated pattern of Leiomyomatosis Peritonealis Disseminata (LPD). Although these experimental nodules were considered to be composed of fibroblasts by light-microscopic studies, LPD was verified to be composed of smooth muscle by electronmicroscopic studies. Therefore, it is indispensable to study these experimental nodules by electronmicroscopy, when we discuss about the similarities between experimental nodules and LPD. Guinea pigs were treated with various doses of estradiol benzoate (E) [40, 80, 100, 200 microgram/day, thrice a week, intramuscular injection]. After treatment for three months, every dose of E produced the lesions simulating the disseminated pattern of LPD: multiple nodules in various sizes, scattered on the surface of the uterine horn, peritoneum, spleen and pancreas, and in the omentum and mesocolon. Ultrastructurally, the nodules were composed of the cells resembling fibroblast. We conjectured that estrogen differentiates and proliferates the cells of the features of fibroblast-like cells from subcoelomic mesenchyme, resulting in the production of multiple superitoneal nodules simulating the disseminated pattern of LPD.
据报道，用大剂量雌激素处理的豚鼠会产生多个腹膜纤维瘤，当停用类固醇刺激物时，这些结节会消退。最近，这些实验性结节被认为类似于播散性腹膜平滑肌瘤病（LPD）的播散模式。尽管通过光学显微镜研究认为这些实验性结节由成纤维细胞组成，但通过电子显微镜研究证实LPD由平滑肌组成。因此，当我们讨论实验性结节与LPD之间的相似性时，通过电子显微镜研究这些实验性结节是必不可少的。用不同剂量的苯甲酸雌二醇（E）[40、80、100、200微克/天，每周三次，肌肉注射]处理豚鼠。治疗三个月后，每剂量的E都会产生模拟LPD播散模式的病变：各种大小的多个结节，散在于子宫角、腹膜、脾脏和胰腺表面，以及大网膜和结肠系膜中。在超微结构上，这些结节由类似于成纤维细胞的细胞组成。我们推测，雌激素使间皮间充质中具有成纤维细胞样细胞特征的细胞分化并增殖，从而产生模拟LPD播散模式的多个腹膜结节。
