Bauer R, Schütz R
Arch Dermatol Res. 1980;269(3):261-8. doi: 10.1007/BF00406419.
Living Sézary cells are more clearly discernible in supravital preparations using a phase contrast microscope than in stained blood smears. Especially, the small variant of the Sézary cell can easily be identified by this simple technique. Lymphoblasts are unequivocally discernible from monocytes, since functional criteria of the living cells can be evaluated in supravital preparations in addition to morphological characteristics. Using this technique a remarkable phenomenon was seen in living Sézary cells: interactions between autologous erythrocytes and the atypical lymphocytes created autologous rosettes. The binding of erythrocytes with a typical lymphocytes was so stable that even with good motility of the Sézary cells the erythrocytes adhered to the surface of the lymphocytes. The ability of living Sézary cells to build rosettes with autologous erythrocytes in vitro is taken as an indication that living Sézary cells have membrane markers of thymocytes.
使用相差显微镜在体外活体标本中比在染色血涂片上更能清晰地辨别活的Sezary细胞。特别是，通过这种简单技术可以很容易地识别出Sezary细胞的小变异型。淋巴母细胞与单核细胞很容易区分，因为除了形态特征外，还可以在体外活体标本中评估活细胞的功能标准。使用这项技术，在活的Sezary细胞中观察到一个显著现象：自体红细胞与非典型淋巴细胞之间的相互作用形成了自体玫瑰花结。红细胞与典型淋巴细胞的结合非常稳定，以至于即使Sezary细胞具有良好的运动性，红细胞仍能附着在淋巴细胞表面。活的Sezary细胞在体外与自体红细胞形成玫瑰花结的能力被视为活的Sezary细胞具有胸腺细胞膜标志物的一个指标。
