分离的肝细胞中线粒体和胞质的ATP/ADP比率：洋地黄皂苷法与非水分离法的比较
Mitochondrial and cytosolic ATP/ADP ratios in isolated hepatocytes. A comparison of the digitonin method and the non-aqueous fractionation procedure.
作者信息
Soboll S, Akerboom T P, Schwenke W D, Haase R, Sies H
出版信息
Biochem J. 1980 Dec 15;192(3):951-4. doi: 10.1042/bj1920951.
Abstract
The ratio of ATP content/ADP content in the mitochondrial matrix was found to be 2.07 +/- 0.21 and 2.26 +/- 0.22 as determined with six different preparations of isolated hepatocytes subfractionated with the digitonin and non-aqueous-fractionation procedures, respectively. In contrast, the mitochondrial matrix ATP/ADP determined with isolated haemoglobin-free perfused liver by using the non-aqueous-fractionation procedure was about 0.2, whereas the cytosolic values obtained with isolated cells and with the intact organ were similar. It is concluded that the relatively higher ATP/ADP ratio in the mitochondrial matrix of isolated hepatocytes represents a biochemical difference due to properties of the model rather than a methodological artifact.
摘要
分别采用洋地黄皂苷和非水相分级分离法对分离的肝细胞进行六次不同的分级分离制备，结果发现线粒体基质中ATP含量与ADP含量的比值分别为2.07±0.21和2.26±0.22。相比之下，采用非水相分级分离法测定无血红蛋白灌注肝分离物的线粒体基质ATP/ADP约为0.2，而分离细胞和完整器官获得的胞质值相似。得出的结论是，分离肝细胞线粒体基质中相对较高的ATP/ADP比值代表了由于模型特性导致的生化差异，而非方法学假象。
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