Berman W F, Rogers S R, Bautista J O, Segal S
Metabolism. 1978 Dec;27(12):1721-31. doi: 10.1016/0026-0495(78)90258-5.
The development of the technique for the perfusion of the immature liver has enabled us to characterize metabolic differences in carbohydrate metabolism in the suckling versus adult rat livers. Livers of fasted suckling and adult rats were perfused with 4 mM galactose or 4 mM glucose. Galactose uptake was the same for both age groups during the first 35 min. The adult liver maintained the initial rate of uptake after this period while the immature liver began to take up galactose more rapidly. By the end of the experimental period, on a weight basis, uptake by the young liver was three times that of the adult. Analysis of the livers at the end of the 90 min perfusion showed hepatic galactose concentrations to be one-half of circulating media levels. Glucose output was observed in each group during perfusion with either galactose or glucose. In the immature liver, galactose perfusion stimulated more glucose output than did the glucose perfusion. In the adult, however, both sugars resulted in the same levels of glucose output. Galactose perfusion resulted in glucose levels in young liver being higher than the media; while in the adult, the level was lower than the media. Galactose perfused livers of the suckling and adult contained significantly more uridine-5'-diphosphogalactose than the glucose perfused livers of each age.
未成熟肝脏灌注技术的发展使我们能够表征乳鼠和成年大鼠肝脏碳水化合物代谢的代谢差异。禁食的乳鼠和成年大鼠的肝脏用4 mM半乳糖或4 mM葡萄糖进行灌注。在最初的35分钟内，两个年龄组的半乳糖摄取量相同。在此之后，成年肝脏维持初始摄取率，而未成熟肝脏开始更快地摄取半乳糖。到实验期结束时，以重量计，幼龄肝脏的摄取量是成年肝脏的三倍。在90分钟灌注结束时对肝脏进行分析，结果显示肝脏半乳糖浓度为循环培养基水平的一半。在用半乳糖或葡萄糖灌注期间，每组均观察到葡萄糖输出。在未成熟肝脏中，半乳糖灌注比葡萄糖灌注刺激更多的葡萄糖输出。然而，在成年肝脏中，两种糖导致相同水平的葡萄糖输出。半乳糖灌注导致幼龄肝脏中的葡萄糖水平高于培养基；而在成年肝脏中，该水平低于培养基。与每个年龄组的葡萄糖灌注肝脏相比，半乳糖灌注的乳鼠和成年大鼠肝脏中含有的尿苷-5'-二磷酸半乳糖明显更多。
