Sparks J W, Lynch A, Chez R A, Glinsmann W H
Pediatr Res. 1976 Jan;10(1):51-6. doi: 10.1203/00006450-197601000-00010.
Glycogen metabolism was studied in the isolated perfused liver of the monkey conceptus at 90% of gestation using an in situ recirculating perfusion system. Net uptake of glucose and galactose and the activities of the enzymes, glycogen synthetase and phosphorylase, were studied in response to varied perfusate composition. Synthetase activity was expressed as %I. the percentage of total synthetase activity in the active form. Perfusate glucose concentrations as high as 700 mg/100 ml did not lead to net glucose uptake of to an increase in the baseline %I synthetase (4 +/- 1, mean +/- SEM). In the presence of 300 mg/100 ml glucose, insulin at 10(-7) M in creased %I to 8 +/- 2, and galactose greater than 75 mg/100 ml increased %I to 8 +/- 1. The combination of galactose, glucose, and insulin increased %I to 40 +/- 5. With this latter combination, synthetase activity was proportional to perfusate glucose concentration above 100 mg/100 ml. Phosphorylase activity was diminished by either galactose or insulin, and phosphorylase activity was lowest in the group receiving galactose, glucose, and insulin. Galactose was taken up by all livers, but net glucose uptake was not observed under any condition; net hexose uptake was observed in perfusions with galactose. Glycogen levels did not vary significantly with varied perfusate composition during the 30-min perfusion periods.
利用原位再循环灌注系统，在妊娠90%的猴胚胎离体灌注肝脏中研究了糖原代谢。研究了葡萄糖和半乳糖的净摄取以及糖原合成酶和磷酸化酶的活性，以响应不同的灌注液组成。合成酶活性以%I表示，即活性形式的总合成酶活性的百分比。高达700mg/100ml的灌注液葡萄糖浓度并未导致葡萄糖的净摄取或基线%I合成酶增加（4±1，平均值±标准误）。在存在300mg/100ml葡萄糖的情况下，10^(-7)M的胰岛素将%I提高到8±2，大于75mg/100ml的半乳糖将%I提高到8±1。半乳糖、葡萄糖和胰岛素的组合将%I提高到40±5。对于后一种组合，合成酶活性与高于100mg/100ml的灌注液葡萄糖浓度成正比。半乳糖或胰岛素均可降低磷酸化酶活性，在接受半乳糖、葡萄糖和胰岛素的组中磷酸化酶活性最低。所有肝脏均摄取半乳糖，但在任何条件下均未观察到葡萄糖的净摄取；在半乳糖灌注中观察到己糖的净摄取。在30分钟的灌注期内，糖原水平随灌注液组成的变化无显著差异。
