Kinsman O S, White M I, Noble W C
Br J Exp Pathol. 1981 Apr;62(2):142-5.
To throw more light on the role of Protein A (a cell-wall component of most strains of S. aureus), in infection and inflammation, due to this organism the immediate inflammatory reaction has been studied in hairless and hairless/obese mice after s.c. injection of the protein into the footpad following various forms of immunization or pretreatment (described). Non-immunized mice showed an inflammatory reaction to Protein A, as judged by swelling, reaching a peak 2 h after injection. This might have been due to a nonspecific interaction between certain mouse Igs and Protein A. When specific antibody levels were raised by prior immunization or infection, the swelling was greatly increased. No delayed reaction was seen at 24 or 48 h, nor was a positive patch test obtained. The difference in results seen in mice and other animals may be due partly to the fact that intradermal injections are not possible in the mouse, or because in the mouse, unlike other subjects which have been used, histamine does not play a part. Mice do not show anaphylactic shock and this may be a function of the class of murine Igs interacting with Protein A. Further studies on these factors are required.
为了更深入了解金黄色葡萄球菌大多数菌株的细胞壁成分A蛋白在感染和炎症中的作用，在以各种形式免疫或预处理（如所述）后，将该蛋白皮下注射到无毛和无毛/肥胖小鼠的足垫中，研究了由该生物体引起的即时炎症反应。未免疫的小鼠对A蛋白表现出炎症反应，通过肿胀判断，注射后2小时达到峰值。这可能是由于某些小鼠免疫球蛋白与A蛋白之间的非特异性相互作用。当通过预先免疫或感染提高特异性抗体水平时，肿胀会大大增加。在24小时或48小时未见迟发性反应，也未获得阳性斑贴试验结果。在小鼠和其他动物中观察到的结果差异可能部分是由于在小鼠中无法进行皮内注射，或者是因为与其他已使用的实验对象不同，组胺在小鼠中不起作用。小鼠不会出现过敏性休克，这可能是与A蛋白相互作用的鼠免疫球蛋白类别所起的作用。需要对这些因素进行进一步研究。
