Bradbury M, Baker N R
Biochim Biophys Acta. 1981 May 13;635(3):542-51. doi: 10.1016/0005-2728(81)90113-4.
An analysis of the photo-induced decline in the in vivo chlorophyll a fluorescence emission (Kautsky phenomenon) from the bean leaf is presented. The redox state of PS II electron acceptors and the fluorescence emission from PS I and PS II were monitored during quenching of fluorescence from the maximum level at P to the steady state level at T. Simultaneous measurement of the kinetics of fluorescence emission associated with PS I and PS II indicated that the ratio of P s I/PS II emission changed in an antiparallel fashion to PS II emission throughout the induction curve. Estimation of the redox state of PS II electron acceptors at given points during P to T quenching was made by exposing the leaf to additional excitation irradiation and determining the amount of variable PS II fluorescence generated. An inverse relationship was found between the proportion of PS II electron acceptors in the oxidised state and PS II fluorescence emission. The interrelationships between the redox state of PS II electron acceptors and fluorescence emission from PS I and PS II remained similar when the shape of the induction curve from P to T was modified by increasing the excitation photon flux density. The contributions of photochemical and nonphotochemical quenching to the in vivo fluorescence decline from P to T are discussed.
本文对菜豆叶片光诱导的体内叶绿素a荧光发射下降（考茨基现象）进行了分析。在荧光从P点的最大水平猝灭至T点的稳态水平的过程中，监测了PS II电子受体的氧化还原状态以及PS I和PS II的荧光发射。对与PS I和PS II相关的荧光发射动力学的同步测量表明，在整个诱导曲线中，P s I/PS II发射比率与PS II发射呈反平行变化。通过使叶片暴露于额外的激发照射并确定产生的可变PS II荧光量，对P至T猝灭期间给定时间点的PS II电子受体氧化还原状态进行了估算。发现处于氧化态的PS II电子受体比例与PS II荧光发射之间呈反比关系。当通过增加激发光子通量密度改变从P到T的诱导曲线形状时，PS II电子受体的氧化还原状态与PS I和PS II荧光发射之间的相互关系保持相似。讨论了光化学猝灭和非光化学猝灭对从P到T的体内荧光下降的贡献。
