Akimenko V K, Medentsev A G
Biokhimiia. 1977 Oct;42(10):1905-9.
The study of reversible inactivation process of alternative pathway of electron transfer in cyanide-resistant mitochondria of C. lipolytica yeast was carried out. The appearance of sensitivity of respiration of cyanide-resistant mitochondria to cyanide and lowering of sensitivity of substrate oxidation to benzohydroxamic acid (the inhibitor of electron transfer along the alternative pathway) during the incubation was observed. The increase of temperature and the presence of magnesium ions in the incubation medium accelerates this process. The loss of cyanide resistance of respiration by mitochondria is not practically followed by the decrease of NADH-and alpha-glycerophosphatoxidase activity. Reactivation of cyanide resistance of respiration in incubated mitochondria is reached during washing them with the medium containing EDTA. The addition of magnesium ions into the washing medium prevents the reactivation process. On the basis of data obtained it is concluded that the relation between the respiratory chain and alternative oxidase is regulated by magnesium.
对解脂耶氏酵母抗氰线粒体中电子传递替代途径的可逆失活过程进行了研究。观察到在孵育过程中，抗氰线粒体呼吸对氰化物的敏感性出现，同时底物氧化对苯甲羟肟酸（沿替代途径的电子传递抑制剂）的敏感性降低。孵育温度的升高和孵育介质中镁离子的存在加速了这一过程。线粒体呼吸抗氰性的丧失实际上并未伴随着NADH和α-甘油磷酸氧化酶活性的降低。在用含有EDTA的介质洗涤孵育的线粒体期间，可实现呼吸抗氰性的重新激活。向洗涤介质中添加镁离子可阻止重新激活过程。根据所获得的数据得出结论，呼吸链与交替氧化酶之间的关系受镁调节。
