Clarke D R, Park J B
Biomaterials. 1981 Jan;2(1):9-13. doi: 10.1016/0142-9612(81)90080-6.
This study was undertaken in order to investigate the use of fluid perfusion to prevent the adhesion of erythrocytes to a porous foreign surface. Three pore sizes each of two different commercially available polyethylene and aluminum oxide (AI2O3) tubes were investigated. Tubes were perfused with Ringer's solution while immersed in a bath of whole canine blood. Control tubes were prepared in an identical fashion but were not perfused. After testing, tubes were fixed in glutaraldehyde and dehydrated with graded ethanol. Samples were then sectioned and prepared for erythrocyte adhesion analysis by scanning electron microscopy (SEM). Results indicate that fluid perfusion can be used as a means to prevent erythrocyte adhesion in the range of pore sizes (10-60 micrometer diameter) studied. Critical values of the fluid perfusion rate are 0.027, 0.073 and 0.21 ml/min. cm2 for 10 micrometer, 35 micrometer, and 60 micrometer pore polyethylene respectively. The critical values for the ceramic samples are 0.22, 0.16 and 0.72 ml/min cm2 respectively for 7, 11 and 31 micrometer pore diameter respectively.
本研究旨在探讨使用流体灌注来防止红细胞黏附于多孔异物表面。研究了两种不同市售聚乙烯管和氧化铝（Al2O3）管的三种孔径。将管子浸入全犬血浴中时用林格氏液灌注。对照管以相同方式制备但不进行灌注。测试后，将管子固定在戊二醛中并用梯度乙醇脱水。然后将样品切片并通过扫描电子显微镜（SEM）制备用于红细胞黏附分析的样本。结果表明，在所研究的孔径范围（直径10 - 60微米）内，流体灌注可作为防止红细胞黏附的一种手段。对于10微米、35微米和60微米孔径的聚乙烯管，流体灌注速率的临界值分别为0.027、0.073和0.21毫升/分钟·平方厘米。对于陶瓷样品，对于孔径分别为7微米、11微米和31微米的情况，临界值分别为0.22、0.16和0.72毫升/分钟·平方厘米。
