Canalis E, Caldarella A M, Reardon G E
Clin Chem. 1981 Jul;27(7):1241-3.
17 alpha-Hydroxyprogesterone, cortisol, and 11-deoxycortisol were extracted with methylene chloride, separated by liquid chromatography, identified by their retention times, and quantitated by monitoring the absorbance at 254 nm. The method is specific and sensitive to as little as 2.5 ng of 17 alpha-hydroxyprogesterone. Mean analytical recovery of added 17 alpha-hydroxyprogesterone was 99% (SD 11%) and the CV for the same or different assays ranged from 3.5 to 9.4%. 6 beta-Hydroxyprogesterone was used as the internal standard. Concentrations of 17 alpha-hydroxyprogesterone in serum of normal men and women were less than 2.5 micrograms/L, but women in the luteal phase of the menstrual cycle had higher values. Mean 17 alpha-hydroxyprogesterone content in serum from mixed cord blood of normal newborns was 28.1 (SD 13.8) micrograms/L. Results by this method correlated well (r = 0.97) with results by radioimmunoassay, but were somewhat higher.
17α-羟孕酮、皮质醇和11-脱氧皮质醇用二氯甲烷萃取，通过液相色谱分离，根据保留时间进行鉴定，并通过监测254nm处的吸光度进行定量。该方法特异性强，对低至2.5ng的17α-羟孕酮也很敏感。添加的17α-羟孕酮的平均分析回收率为99%（标准差11%），相同或不同测定的变异系数范围为3.5%至9.4%。6β-羟孕酮用作内标。正常男性和女性血清中17α-羟孕酮的浓度低于2.5μg/L，但处于月经周期黄体期的女性值较高。正常新生儿混合脐血血清中17α-羟孕酮的平均含量为28.1（标准差13.8）μg/L。该方法的结果与放射免疫分析的结果相关性良好（r = 0.97），但略高。
