Blacklock E C, Sadler P A
Clin Chim Acta. 1981 Jun 2;113(1):87-94. doi: 10.1016/0009-8981(81)90443-5.
A semi-quantitative screening method for heavy metals in biological material is described. The metals are complexed with ammonium pyrrolidine dithiocarbamate, sodium diethyl dithiocarbamate and potassium sodium tartrate. The solutions are adjusted to pH 4 and then extracted into chloroform. The chloroform phase is evaporated onto a matrix mixture of lithium fluoride and graphite. The sample is analysed by direct current arc emission spectroscopy using a 3 metre grating spectrograph. The spectra are recorded on a photographic plate. The method is developed on aqueous and spiked samples and then applied to in vivo samples containing toxic levels of heavy metals. Atomic absorption spectroscopy is used to check standard concentrations and to monitor the efficiency of the extraction procedure.
本文描述了一种用于生物材料中重金属的半定量筛选方法。金属与吡咯烷二硫代氨基甲酸铵、二乙基二硫代氨基甲酸钠和酒石酸钠钾络合。将溶液调节至pH 4，然后萃取到氯仿中。将氯仿相蒸发到氟化锂和石墨的基质混合物上。使用3米光栅光谱仪通过直流电弧发射光谱法对样品进行分析。光谱记录在照相板上。该方法先在水样和加标样品上进行开发，然后应用于含有毒性水平重金属的体内样品。使用原子吸收光谱法检查标准浓度并监测萃取程序的效率。
