Johansson B B
Exp Brain Res. 1981;42(3-4):331-6. doi: 10.1007/BF00237498.
The experiments were performed to determine if indomethacin, a prostaglandin synthesis inhibitor, could reduce albumin extravasation and brain edema in some models of blood-brain barrier dysfunction. The blood pressure was increased by i.v. adrenaline or bicuculline in conscious rats with indwelling catheters in the aorta and jugular vein. 125I-labeled serum albumin and Evans blue-albumin were used as tracers of the blood-brain barrier function. Pretreatment with indomethacin significantly reduced albumin extravasation after the administration of adrenalin but not after bicuculline, i.e. when acute hypertension was combined with a metabolically mediated cerebral vasodilatation. It is argued that the protective effect of indomethacin in adrenaline-induced hypertension probably is related to the vasoconstrictory effect of the drug. Five microliters of air or Lipiodol were injected into the right internal carotid artery in rats anesthetized with pentobarbitone. The albumin content in the injected hemisphere was seven to nine times higher after fat than after air embolism. No significant reduction of tracer extravasation was obtained in rats treated with indomethacin. Rats subjected to fat embolism had a significant homolateral cerebral edema (i.e. increased water content) which was not reduced by pretreatment with indomethacin. By contrast, the water content was significantly increased also in the non-injected side in rats given indomethacin indicating a larger spread of edema fluid in these animals.
进行这些实验是为了确定前列腺素合成抑制剂吲哚美辛是否能在某些血脑屏障功能障碍模型中减少白蛋白外渗和脑水肿。在主动脉和颈静脉留置导管的清醒大鼠中，通过静脉注射肾上腺素或荷包牡丹碱升高血压。使用125I标记的血清白蛋白和伊文思蓝白蛋白作为血脑屏障功能的示踪剂。吲哚美辛预处理显著降低了肾上腺素给药后的白蛋白外渗，但在荷包牡丹碱给药后未降低，即当急性高血压与代谢介导的脑血管扩张同时存在时。有人认为，吲哚美辛在肾上腺素诱导的高血压中的保护作用可能与该药物的血管收缩作用有关。在戊巴比妥麻醉的大鼠中，将5微升空气或碘油注入右颈内动脉。脂肪栓塞后注射侧半球的白蛋白含量比空气栓塞后高7至9倍。用吲哚美辛治疗的大鼠中，示踪剂外渗没有显著减少。脂肪栓塞的大鼠出现明显的同侧脑水肿（即含水量增加），吲哚美辛预处理并未使其减轻。相比之下，给予吲哚美辛的大鼠未注射侧的含水量也显著增加，表明这些动物中水肿液的扩散范围更大。
