Thomas N, Bell P A
Mol Cell Endocrinol. 1981 Apr;22(1):71-84. doi: 10.1016/0303-7207(81)90103-9.
The events preceding glucocorticoid-induced lymphocytolysis have been studied in isolated rat thymocytes. Incubation of thymocytes at 37 degrees C in the presence of 1 microM dexamethasone resulted in the progressive appearance of pyknotic cells of modal diameter 4.5 mum, distinct from normal cells of diameter 5.2 mum. The rate of appearance of the pyknotic cells was determined by selective electronic cell counting, and was shown to be accompanied by increased nuclear fragility. The production of pyknotic cells was glucocorticoid-specific, dose-dependent, blocked by cycloheximide, and preceded the loss of cell viability as determined by dye exclusion. The pyknotic cells were separated from the non-pyknotic cells by density gradient centrifugation and shown to be solely responsible for the observed nuclear fragility.
在分离的大鼠胸腺细胞中研究了糖皮质激素诱导淋巴细胞溶解之前的事件。在1微摩尔地塞米松存在下于37℃孵育胸腺细胞，导致逐渐出现核固缩细胞，其平均直径为4.5微米，与直径5.2微米的正常细胞不同。通过选择性电子细胞计数确定核固缩细胞的出现速率，并显示其伴随着核脆性增加。核固缩细胞的产生具有糖皮质激素特异性、剂量依赖性，被环己酰亚胺阻断，并且在通过染料排除法测定的细胞活力丧失之前出现。通过密度梯度离心将核固缩细胞与非核固缩细胞分离，并显示其是观察到的核脆性的唯一原因。
