Minamide S, Naora H, Adachi M, Okano A, Naora H
Japan Immunoresearch Laboratories, Gunma Prefecture.
Histochem Cell Biol. 1995 May;103(5):339-43. doi: 10.1007/BF01457808.
Skin renewal is a typical example of the active participation of a cell in its own death process. Cells arising from mitotic activity in the stratum germinativum of the epidermis continuously migrate upwards to the stratum corneum, where dead cells are eventually desquamated. Recent studies have suggested that apoptosis is involved in the dynamic process of skin renewal. However, this still remains to be further elucidated. In this paper, we investigated the involvement of apoptosis in the skin renewal process. Changes in the morphology of cells in different epidermal layers were compared with histochemical analyses of the extent of DNA fragmentation, as determined by nick end-labelling, and of the reactivities to a monoclonal antibody directed to Le(y)-antigen, difucosylated type 2 chain determinant, which has a close association with apoptosis, and to a monoclonal antibody directed to the proliferating cell nuclear antigen. The results show that apoptosis proceeds concomitantly with cell movement in the epidermis. It seems likely that commitment of a cell to death by apoptosis occurs in the epidermal tissue immediately after completion of cell proliferation, and that Le(y)-antigen expression may be involved in the entire apoptotic process including this early event.
皮肤更新是细胞积极参与自身死亡过程的一个典型例子。由表皮生发层有丝分裂活动产生的细胞不断向上迁移至角质层，最终死细胞在角质层脱落。最近的研究表明，细胞凋亡参与了皮肤更新的动态过程。然而，这仍有待进一步阐明。在本文中，我们研究了细胞凋亡在皮肤更新过程中的作用。通过切口末端标记法测定不同表皮层细胞形态的变化，并与DNA片段化程度的组织化学分析进行比较，同时分析了与细胞凋亡密切相关的针对二岩藻糖基化2型链决定簇Le(y)抗原的单克隆抗体以及针对增殖细胞核抗原的单克隆抗体的反应性。结果表明，细胞凋亡与表皮中的细胞移动同时进行。细胞增殖完成后，表皮组织中细胞可能立即通过细胞凋亡走向死亡，并且Le(y)抗原的表达可能参与包括这一早期事件在内的整个凋亡过程。
