非糖尿病和糖尿病黑冠猕猴体内的免疫反应性胰高血糖素

Immunoreactive glucagon in nondiabetic and diabetic Macaca nigra.

作者信息

Howard C F, Van Bueren A

出版信息

Horm Metab Res. 1981 Apr;13(4):203-6. doi: 10.1055/s-2007-1019221.

DOI:10.1055/s-2007-1019221

Abstract

The primary form of immunoreactive glucagon (IRG) in Macaca nigra has been identified as pancreatic, alpha-cell-size glucagon (IRG3500), with a molecular weight of about 3500. Assays with 30K and K-964 glucagon antibodies gave virtually identical results. Column chromatography of plasma on Bio-Gel P-30 indicated ony minimal amounts of high-molecular-weight IRG. Levels of IRG decrease during a glucose infusion, a response expected of IRG3500. IRG concentrations apparently greater than human values appear to be characteristic of nonhuman primates. Nondiabetic Macaca nigra average 641 pg of IRG3500/ml. Borderline diabetic monkeys with moderately increased glucose and impaired glucose clearance average 2,938 pg/ml. Diabetic monkeys with hyperglycemia and diminished glucose clearance have 375 pg of IRG3500/ml. Changes in IRG3500 are related to a lesion in the islets of Langerhans.

摘要

黑猕猴体内免疫反应性胰高血糖素（IRG）的主要形式已被鉴定为胰腺α细胞大小的胰高血糖素（IRG3500），分子量约为3500。用30K和K - 964胰高血糖素抗体进行检测得到了几乎相同的结果。在Bio - Gel P - 30上对血浆进行柱色谱分析表明，高分子量IRG的含量极少。在葡萄糖输注过程中，IRG水平会下降，这是IRG3500预期的反应。明显高于人类值的IRG浓度似乎是非人类灵长类动物的特征。非糖尿病黑猕猴的IRG3500平均为641 pg/ml。血糖适度升高且葡萄糖清除受损的边缘性糖尿病猴平均为2938 pg/ml。患有高血糖症且葡萄糖清除能力下降的糖尿病猴的IRG3500为375 pg/ml。IRG3500的变化与胰岛病变有关。