Outzen H C
Int J Cancer. 1980 Jul 15;26(1):87-92. doi: 10.1002/ijc.2910260114.
The incidence of tumor formation in MCA-treated skin grafted onto maximally immunosuppressed mice that had been restored to varying extents with normal spleen cells was significantly greater in the mice with intermediate immune capacities than in those that had either minimal or maximal capacities. A similar biphasic tumor incidence curve was observed when MCA-treated skin was grafted onto mice of varying immune capacities, produced by thymectomy and varying doses of whole-body irradiation. Significantly more tumors occurred in the mice given moderate doses of irradiation than in tohse given higher or lower doses. That both of these procedures were actually able to induce discrete levels of immunocompetence was demonstrated by measuring skin allograft rejection times. The immunomodulated mice were observed to have skin graft rejection times which strongly correlated with the number of immunologically competent spleen cells transferred into them. The outgrowth potential of syngeneic normal mammary epithelial cells grafted into cleared mammary fat pads was similar in both immunologically altered and normal control mice, showing that immunoaltered and normal control mice were equally able to support the growth of transplanted normal tissues. These results, which conform with the predictions of the immunostimulation hypothesis, suggest that the immune response is able to stimulate as well as inhibit oncogenesis.
将经甲基胆蒽（MCA）处理的皮肤移植到已用正常脾细胞不同程度恢复的最大程度免疫抑制小鼠身上，免疫能力处于中等水平的小鼠中肿瘤形成的发生率显著高于免疫能力最低或最高的小鼠。当将经MCA处理的皮肤移植到通过胸腺切除和不同剂量全身照射产生的具有不同免疫能力的小鼠身上时，观察到了类似的双相肿瘤发生率曲线。接受中等剂量照射的小鼠中出现的肿瘤明显多于接受高剂量或低剂量照射的小鼠。通过测量皮肤同种异体移植排斥时间证明，这两种方法实际上都能够诱导不同水平的免疫能力。观察到免疫调节小鼠的皮肤移植排斥时间与转移到它们体内的免疫活性脾细胞数量密切相关。将同基因正常乳腺上皮细胞移植到清除的乳腺脂肪垫中，在免疫改变的小鼠和正常对照小鼠中的生长潜力相似，这表明免疫改变的小鼠和正常对照小鼠同样能够支持移植的正常组织生长。这些结果与免疫刺激假说的预测相符，表明免疫反应既能刺激也能抑制肿瘤发生。
