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通过对流免疫电泳在血清中形成非特异性沉淀素。

Formation of nonspecific precipitants in sera by counterimmunoelectrophoresis.

作者信息

Chan J K, Folds J D

出版信息

J Clin Microbiol. 1981 May;13(5):877-9. doi: 10.1128/jcm.13.5.877-879.1981.

DOI:10.1128/jcm.13.5.877-879.1981
PMID:7240399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273907/
Abstract

Formation of nonspecific precipitants in sera in different kinds of agarose and batches of barbital buffer by routine counterimmunoelectrophoresis was observed. These nonspecific precipitants consistently locating at the cathodal side of the well upon counterimmunoelectrophoresis can lead to the incorrect interpretation that a specific antibody is present in the patient's serum against the antigens tested. This observation indicates the importance of including a negative control (patient's serum alone) with each individual sample tested by counterimmunoelectrophoresis for the detection of specific antibody to reduce false-positives.

摘要

通过常规对流免疫电泳观察到，在不同种类的琼脂糖和巴比妥缓冲液批次中，血清中会形成非特异性沉淀剂。这些非特异性沉淀剂在对流免疫电泳时始终位于孔的阴极侧，可能导致错误地认为患者血清中存在针对所检测抗原的特异性抗体。这一观察结果表明，在通过对流免疫电泳检测特异性抗体时，对每个检测样本都包含阴性对照（仅患者血清）以减少假阳性的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ba2/273907/6905d5890dd9/jcm00166-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ba2/273907/6905d5890dd9/jcm00166-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ba2/273907/6905d5890dd9/jcm00166-0096-a.jpg

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本文引用的文献

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Teichoic acid antibodies in staphylococcal and nonstaphylococcal endocarditis.葡萄球菌性和非葡萄球菌性心内膜炎中的磷壁酸抗体。
Ann Intern Med. 1972 Jul;77(1):87-90. doi: 10.7326/0003-4819-77-1-87.
2
Teichoic acid serologic diagnosis of staphylococcal endocarditis. Use of gel diffusion and counterimmunoelectrophoretic methods.葡萄球菌性心内膜炎的磷壁酸血清学诊断。凝胶扩散法和对流免疫电泳法的应用。
Ann Intern Med. 1975 Jan;82(1):13-7. doi: 10.7326/0003-4819-82-1-13.
3
Teichoic acid antibodies in the diagnosis of serious infections with Staphylococcus aureus.
磷壁酸抗体在金黄色葡萄球菌严重感染诊断中的应用
Ann Intern Med. 1976 May;84(5):543-6. doi: 10.7326/0003-4819-84-5-543.
4
Teichoic acid antibody test: its use in patients with coagulase-positive staphylococcal bacteremia.磷壁酸抗体检测：其在凝固酶阳性葡萄球菌菌血症患者中的应用。
Arch Intern Med. 1977 Nov;137(11):1522-5. doi: 10.1001/archinte.137.11.1522.
5
Counterimmunoelectrophoretic detection of a high incidence of precipitin reactions in normal human sera against staphylococcal teichoic acids and protein A.对流免疫电泳检测正常人血清中针对葡萄球菌磷壁酸和蛋白A的沉淀素反应高发率。
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6
Teichoic acid antibodies in chronic staphylococcal osteomyelitis.慢性葡萄球菌骨髓炎中的磷壁酸抗体。
Ann Intern Med. 1978 Oct;89(4):494-6. doi: 10.7326/0003-4819-89-4-494.
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Solid-phase radioimmunoassay for immunoglobulin G Staphylococcus aureus antibody in serious staphylococcal infection.用于检测严重葡萄球菌感染中免疫球蛋白G型金黄色葡萄球菌抗体的固相放射免疫测定法
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