Miyagami J, Matsuhashi K, Kanazawa M, Yanaihara T, Nakayama T
Nihon Sanka Fujinka Gakkai Zasshi. 1980 Jan;32(1):19-26.
The concentrations of immunoreactive secretin (S) and gastrin (G) in maternal peripheral vein (MV) during pregnancy and MV, umbilical artery (UA) and umbilical vein (UV) at delivery and fetal tissue of 19, 23 weeks of gestation obtained after therapeutic abortion were measured by specific radioimmunoassay. S-specific antisera was generated in rabbits against highly purified synthetic S. Newly synthesized S analog (Tyr1)-S was indicated by enzymic method. The radioimmunoassay of S was performed by double antibody method using synthetic S as standard. G was measured by RIA kit (Dainabot radioisotope Lab.). The sensitivity of the assays for S and G was 50 pg/ml. The fetal tissue were extracted by water-boiling method and purified by gel filtration on Sephadex G-25 column. The S levels in MV increase as gestational age advances and reach to the maximum levels of 248 +/- 150 pg/ml, while no increase tendency was noticed in G levels. The S levels in MV, UA and UV at delivery (n = 18) were 307 +/- 158 pg/ml, 460 +/- 166 pg/ml and 424 +/- 126 pg/ml, respectively. The S levels in cord blood is statistically higher than that in MV (p less than 0.05). The G levels in MV, UA and UV were 67 +/- 22 pg/ml, 101 +/- 63 pg/ml and 91 +/- 38 pg/ml. The differences of the values between MV and cord blood are significant (p less than 0.05). S like material in fetal upper gastrointestinal tracts was found high in concentration. The extract of fetal duodenum showed a displacement curve paralleled to that of the standard S. In addition, relatively high concentration of immunoreactive S was found in cerebral cortex obtained from fetus of 23 weeks of gestation. G concentration in fetal tissue was also demonstrated. The evidence of immunoreactive S and G in fetal blood and fetal gastrointestinal tracts was reported. These results may be of importance to provide the information in understanding the physiology of the fetus.
采用特异性放射免疫分析法，测定了孕期母体外周静脉（MV）以及分娩时MV、脐动脉（UA）、脐静脉（UV）中的免疫反应性促胰液素（S）和胃泌素（G）的浓度，并对治疗性流产后获得的妊娠19、23周胎儿组织进行了检测。用高度纯化的合成促胰液素免疫家兔，制备促胰液素特异性抗血清。用酶法鉴定新合成的促胰液素类似物（Tyr1）-S。以合成促胰液素为标准品，采用双抗体法进行促胰液素的放射免疫分析。胃泌素用放射免疫分析试剂盒（Dainabot放射性同位素实验室）测定。促胰液素和胃泌素检测的灵敏度均为50 pg/ml。胎儿组织采用水煮法提取，经Sephadex G-25柱凝胶过滤纯化。母体外周静脉中促胰液素水平随孕周增加而升高，最高可达248±150 pg/ml，而胃泌素水平未见升高趋势。分娩时（n = 18）母体外周静脉、脐动脉和脐静脉中的促胰液素水平分别为307±158 pg/ml、460±166 pg/ml和424±126 pg/ml。脐血中促胰液素水平显著高于母体外周静脉（p < 0.05）。母体外周静脉、脐动脉和脐静脉中的胃泌素水平分别为67±22 pg/ml、101±63 pg/ml和91±38 pg/ml。母体外周静脉与脐血中胃泌素水平差异有统计学意义（p < 0.05）。发现胎儿上消化道中促胰液素样物质浓度较高。胎儿十二指肠提取物显示出与标准促胰液素平行的置换曲线。此外，在妊娠23周胎儿的大脑皮层中发现了相对较高浓度的免疫反应性促胰液素。同时也证实了胎儿组织中胃泌素的浓度。报道了胎儿血液和胎儿胃肠道中免疫反应性促胰液素和胃泌素的证据。这些结果对于了解胎儿生理学提供信息可能具有重要意义。
