Hron W T, Menahan L A
J Lipid Res. 1981 Feb;22(2):377-81.
A sensitive micromethod for the colorimetric determination of free fatty acids (FFA) in plasma has been developed. FFA are extracted with chloroform-heptane-methanol with silicic acid being added to eliminate interfering phospholipids. Diphenylcarbazide containing diphenylcarbazone has been chosen as color-developing reagent, since the chromophore formed in the presence of FFA-Cu complex is stable and sensitive. Comparison of the proposed method and a non-routine reference procedure involving, among other steps, isolation of FFA on thin-layer chromatography would indicate that the efficiency of extraction of FFA in the proposed method was 90% that of the reference procedure. The proposed method offers the sensitivity, convenience, and accuracy needed for the determination of FFA in situations where the size of sample available is limiting, as with small rodents.
已开发出一种用于比色法测定血浆中游离脂肪酸（FFA）的灵敏微量方法。用氯仿 - 庚烷 - 甲醇提取FFA，并加入硅酸以消除干扰性磷脂。选择含二苯卡巴腙的二苯碳酰二肼作为显色剂，因为在FFA - 铜络合物存在下形成的发色团稳定且灵敏。将所提出的方法与一种非常规参考方法进行比较，该参考方法在其他步骤中包括在薄层色谱上分离FFA，结果表明所提出方法中FFA的提取效率是参考方法的90%。所提出的方法提供了在样品量有限（如小型啮齿动物）的情况下测定FFA所需的灵敏度、便利性和准确性。
