Holder A T, Spencer E M, Preece M A
J Endocrinol. 1981 May;89(2):275-82. doi: 10.1677/joe.0.0890275.
The growth-promoting effects of a partially purified preparation of somatomedin (12.7 units/mg) were compared with those of various doses of bovine GH (5, 20 and 80 micrograms/day) when injected into hypopituitary dwarf mice. Growth parameters studied were body-weight and tail-length velocities (calculated as the slope of a regression line fitted to daily measurements against time), uptake of 35SO4(2-) into costal cartilage in vivo and organ weights (heart, liver and kidney). In the first experiment somatomedin (6.4 units/day), bovine GH and 0.9% NaCl were injected once daily in a volume of 0.1 ml for 10 days. Treatment with bovine GH promoted a significant dose-dependent increase in body-weight and tail-length velocities and 35SO4(2-) uptake into costal cartilage in vivo. Somatomedin also promoted a significant increase in body-weight velocity and 35SO4(2-) uptake, both responses were between that observed with the lowest dose of bovine GH and control values. Somatomedin did not promote increase in tail-length velocity. Organ weights did not differ significantly between any of the treatment groups when expressed as mg/g body weight. In the second experiment somatomedin (a daily total of 21.6 units/day) and 0.9% NaCl were injected three times per day in a volume of 0.033 ml, bovine GH was again injected once daily in a volume of 0.1 ml, and the treatment period was 12 days. As in the first experiment all doses of bovine GH and somatomedin promoted a significant increase in body-weight velocity. These results are consistent with the somatomedin hypothesis.
将一种部分纯化的生长调节素制剂（12.7单位/毫克）与不同剂量的牛生长激素（5、20和80微克/天）注射到垂体功能减退的侏儒小鼠体内，比较它们的促生长效果。所研究的生长参数包括体重和尾巴长度增长速度（计算为根据每日测量值与时间拟合的回归线斜率）、体内35SO4(2-)进入肋软骨的摄取量以及器官重量（心脏、肝脏和肾脏）。在第一个实验中，每天一次注射生长调节素（6.4单位/天）、牛生长激素和0.9%氯化钠，体积为0.1毫升，持续10天。用牛生长激素治疗可使体重和尾巴长度增长速度以及体内35SO4(2-)进入肋软骨的摄取量显著呈剂量依赖性增加。生长调节素也可使体重增长速度和35SO4(2-)摄取量显著增加，这两种反应均介于最低剂量牛生长激素组与对照组之间。生长调节素未促进尾巴长度增长速度增加。以毫克/克体重表示时，各治疗组之间的器官重量无显著差异。在第二个实验中，每天三次注射生长调节素（每天总量为21.6单位/天）和0.9%氯化钠，体积为0.033毫升，再次每天一次注射牛生长激素，体积为0.1毫升，治疗期为12天。与第一个实验一样，所有剂量的牛生长激素和生长调节素均促进体重增长速度显著增加。这些结果与生长调节素假说一致。
