非洲爪蟾蝌蚪角膜、虹膜和视网膜晶状体形成能力的实验分析。

Experimental analysis of the lens-forming competence of the cornea, iris, and retina in Xenopus laevis tadpoles.

作者信息

Bosco L, Filoni S, Paglioni S

出版信息

J Exp Zool. 1981 May;216(2):267-76. doi: 10.1002/jez.1402160207.

Abstract

The present research was carried out using Xenopus laevis tadpoles at stage 50-51 to ascertain whether eye territories other than the outer cornea and the pericorneal epidermis have lens-forming competence, particularly the iris and/or retina. Five experiments were performed: simple lentectomy; removal of lens, outer cornea, and pericorneal integument; removal of lens, outer cornea, and pericorneal integument, and simultaneous incision of retina; removal of lens, outer cornea and pericorneal integument, and simultaneous incision of dorsal iris; removal of lens and simultaneous lesion of iris and retina. Results show that when lentectomy is performed without damaging any territories other than the outer and inner cornea, lens regeneration is always from the outer cornea, and the iris and retina do not exhibit any lens-forming competence. The iris and retina show no clear-cut lens-forming competence even after they have been stimulated by comparatively extensive lesions.

摘要

本研究使用处于50-51期的非洲爪蟾蝌蚪来确定除角膜外层和角膜周表皮之外的眼组织区域是否具有晶状体形成能力，特别是虹膜和/或视网膜。进行了五项实验：单纯晶状体切除术；切除晶状体、角膜外层和角膜周被膜；切除晶状体、角膜外层和角膜周被膜，并同时切开视网膜；切除晶状体、角膜外层和角膜周被膜，并同时切开背侧虹膜；切除晶状体并同时损伤虹膜和视网膜。结果表明，当进行晶状体切除术而不损伤角膜外层和内层以外的任何组织区域时，晶状体总是从角膜外层再生，虹膜和视网膜不表现出任何晶状体形成能力。即使在受到相对广泛的损伤刺激后，虹膜和视网膜也没有明显的晶状体形成能力。