Bopp R J, Farid K Z, Nash J F
J Pharm Sci. 1981 May;70(5):507-9. doi: 10.1002/jps.2600700511.
A high-performance liquid chromatographic method is described for the quantitation of fenoprofen, dl-2-(3-phenoxyphenyl)-propionic acid, in human plasma. The proteins in plasma were precipitated by the addition of hydrochloric acid. Fenoprofen and the internal standard, dl-2-(4-phenoxyphenyl)valeric acid, were extracted into butyl chloride and then back-extracted into sodium hydroxide. The aqueous solution was injected onto a reversed-phase alkylphenyl column, and the compounds were eluted using a mobile phase of acetonitrile-water-acetic acid (50:50:2 v/v/v). At a flow rate of 1 ml/min, the retention times of fenoprofen and the internal standard were 8 and 12 min, respectively. The absorbance was monitored at 272 nm. The method requires 1.0 ml of plasma and is sensitive to 0.5 microgram/ml. This procedure has been used for routine assay of multiple samples from bioavailability and compliance studies.
本文描述了一种用于定量测定人血浆中苯氧布洛芬（dl-2-(3-苯氧基苯基)丙酸）的高效液相色谱法。通过加入盐酸使血浆中的蛋白质沉淀。苯氧布洛芬和内标物dl-2-(4-苯氧基苯基)戊酸被萃取到氯丁烷中，然后再反萃取到氢氧化钠中。将水溶液注入反相烷基苯基柱，使用乙腈-水-乙酸（50:50:2 v/v/v）的流动相洗脱化合物。流速为1 ml/min时，苯氧布洛芬和内标的保留时间分别为8分钟和12分钟。在272 nm处监测吸光度。该方法需要1.0 ml血浆，灵敏度为0.5微克/毫升。此方法已用于生物利用度和顺应性研究中多个样品的常规测定。
