Sallustio B C, Meffin P J, Thompson M
Department of Clinical Pharmacology, Flinders Medical Centre, Bedford Park, Australia.
J Chromatogr. 1987 Nov 27;422:33-41. doi: 10.1016/0378-4347(87)80437-1.
A normal-phase high-performance liquid chromatographic (HPLC) method has been developed for the quantitation of radiolabelled triacylglycerols containing fenoprofen, synthesized from [3H]glycerol by isolated hepatocytes and adipocytes. The assay consists of extracting the lipids into diethyl ether, separating triacylglycerols from polar endogenous lipids using silica Sep-Pak cartridges and quantitating endogenous triacylglycerols and triacylglycerols containing fenoprofen by HPLC resolution and scintillation counting. HPLC separation is achieved in less than 10 min. Using [14C]tripalmitin as internal standard the assay has a linear relationship between added triacylglycerol and measured endogenous triacylglycerols and triacylglycerols containing fenoprofen with regression coefficients of 0.997 and 0.998, respectively.
已开发出一种正相高效液相色谱（HPLC）方法，用于定量分析由分离的肝细胞和脂肪细胞从[3H]甘油合成的含非诺洛芬的放射性标记三酰甘油。该分析方法包括将脂质提取到乙醚中，使用硅胶Sep-Pak柱从极性内源性脂质中分离出三酰甘油，并通过HPLC分离和闪烁计数对内源性三酰甘油和含非诺洛芬的三酰甘油进行定量。HPLC分离在不到10分钟内完成。使用[14C]三棕榈精作为内标，添加的三酰甘油与测得的内源性三酰甘油和含非诺洛芬的三酰甘油之间具有线性关系，回归系数分别为0.997和0.998。
