Delbeke F T, Debackere M
Faculteit Diergeneeskunde, Universiteit Gent, Belgium.
Biomed Chromatogr. 1994 Jan-Feb;8(1):29-31. doi: 10.1002/bmc.1130080108.
A high performance liquid chromatographic method to measure plasma and urine fenoprofen levels in equine biofluids is described. Liquid-liquid extraction with diethylether was used to isolate the drug from plasma and urine. The accuracy and reproducibility of the method were within acceptable limits over the concentration range 0-10 micrograms/mL and 0-20 micrograms/mL respectively from plasma and urine. Detection limits were 0.05 microgram/mL (2 mL plasma) and 0.2 microgram/mL (0.5 mL urine). This procedure was applied to ascertain the pharmacokinetics of a 3 g dose of fenoprofen calcium in a horse.
本文描述了一种用于测定马生物流体中血浆和尿液中苯氧苯丙酸水平的高效液相色谱法。采用乙醚液-液萃取法从血浆和尿液中分离药物。该方法在血浆浓度范围为0-10微克/毫升、尿液浓度范围为0-20微克/毫升时，其准确性和重现性均在可接受范围内。检测限分别为0.05微克/毫升（2毫升血浆）和0.2微克/毫升（0.5毫升尿液）。该方法用于确定一匹马静脉注射3克苯氧苯丙酸钙后的药代动力学。
