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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Starkey P M, Barrett A J

Biochem J. 1976 May 1;155(2):273-8. doi: 10.1042/bj1550273.

The specificity of cathepsin G, a neutral proteinase from human spleen, was examined by use of low-molecular-weight substrates. The enzyme was found to hydrolyse several synthetic substrates also hydrolysed by chymotrypsin, but with different kinetic constants. 2. Maximal activity against benzoyl-DL-phenylalanine 2-naphthol ester and azo-casein was in the range pH 7.5-8.0. 3. The sensitivity of cathepsin G to the action of potential inhibitors was determined, and compared with those of bovine chymotrypsin and subtilisin. Cathepsin G showed the characteristics of a serine proteinase, but was less affected by the chloromethyl ketone of tosylphenylalanine than was chymotrypsin. 4. A rabbit anti-(human cathepsin G) serum was raised, and precipitin lines formed in agarose gel were stained for activity of the enzyme. 5. Cathepsin G was shown to be immunologically identical with the chymotrypsin-like enzyme of the azurophil granules of the neutrophil granulocytes.

利用低分子量底物对人脾脏中的中性蛋白酶组织蛋白酶G的特异性进行了研究。发现该酶能水解几种也能被胰凝乳蛋白酶水解的合成底物，但动力学常数不同。2. 对苯甲酰-DL-苯丙氨酸2-萘酯和偶氮酪蛋白的最大活性在pH 7.5 - 8.0范围内。3. 测定了组织蛋白酶G对潜在抑制剂作用的敏感性，并与牛胰凝乳蛋白酶和枯草杆菌蛋白酶进行了比较。组织蛋白酶G表现出丝氨酸蛋白酶的特性，但与胰凝乳蛋白酶相比，对甲苯磺酰苯丙氨酸氯甲基酮的影响较小。4. 制备了兔抗（人组织蛋白酶G）血清，并对琼脂糖凝胶中形成的沉淀线进行酶活性染色。5. 结果表明，组织蛋白酶G与中性粒细胞嗜天青颗粒中的类胰凝乳蛋白酶在免疫上相同。

Starkey P M, Barrett A J

Biochem J. 1976 May 1;155(2):273-8. doi: 10.1042/bj1550273.

The specificity of cathepsin G, a neutral proteinase from human spleen, was examined by use of low-molecular-weight substrates. The enzyme was found to hydrolyse several synthetic substrates also hydrolysed by chymotrypsin, but with different kinetic constants. 2. Maximal activity against benzoyl-DL-phenylalanine 2-naphthol ester and azo-casein was in the range pH 7.5-8.0. 3. The sensitivity of cathepsin G to the action of potential inhibitors was determined, and compared with those of bovine chymotrypsin and subtilisin. Cathepsin G showed the characteristics of a serine proteinase, but was less affected by the chloromethyl ketone of tosylphenylalanine than was chymotrypsin. 4. A rabbit anti-(human cathepsin G) serum was raised, and precipitin lines formed in agarose gel were stained for activity of the enzyme. 5. Cathepsin G was shown to be immunologically identical with the chymotrypsin-like enzyme of the azurophil granules of the neutrophil granulocytes.

利用低分子量底物对人脾脏中的中性蛋白酶组织蛋白酶G的特异性进行了研究。发现该酶能水解几种也能被胰凝乳蛋白酶水解的合成底物，但动力学常数不同。2. 对苯甲酰-DL-苯丙氨酸2-萘酯和偶氮酪蛋白的最大活性在pH 7.5 - 8.0范围内。3. 测定了组织蛋白酶G对潜在抑制剂作用的敏感性，并与牛胰凝乳蛋白酶和枯草杆菌蛋白酶进行了比较。组织蛋白酶G表现出丝氨酸蛋白酶的特性，但与胰凝乳蛋白酶相比，对甲苯磺酰苯丙氨酸氯甲基酮的影响较小。4. 制备了兔抗（人组织蛋白酶G）血清，并对琼脂糖凝胶中形成的沉淀线进行酶活性染色。5. 结果表明，组织蛋白酶G与中性粒细胞嗜天青颗粒中的类胰凝乳蛋白酶在免疫上相同。