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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Starkey P M, Barrett A J

Biochem J. 1976 May 1;155(2):265-71. doi: 10.1042/bj1550265.

The elastase of human spleen was shown to exhibit endopeptidase activity against azo-casein and elastin. 2. Activity against several synthetic substrates was detected, and benzyloxycarbonyl-L-alanine 2-naphthyl ester was found to be a good substrate for routine use. 3. The enzyme showed a broad pH optimum in the range of 8.2-9.2 against azo-casein and the synthetic substrate. 4. The effect of inhibitors on the spleen elastase showed it to be a serine proteinase with a specificity similar to that of porcine pancreatic elastase. 5. Specific antisera were raised against the enzyme, and it was shown to be immunologically identical with the lysosomal elastase of human neutrophil leucocytes.

已证明人脾脏弹性蛋白酶对偶氮酪蛋白和弹性蛋白具有内肽酶活性。2. 检测到其对几种合成底物的活性，并且发现苄氧羰基-L-丙氨酸2-萘酯是常规使用的良好底物。3. 该酶在8.2 - 9.2范围内对偶氮酪蛋白和合成底物表现出较宽的pH最适值。4. 抑制剂对脾脏弹性蛋白酶的作用表明它是一种丝氨酸蛋白酶，其特异性与猪胰弹性蛋白酶相似。5. 制备了针对该酶的特异性抗血清，并且证明它与人类嗜中性白细胞的溶酶体弹性蛋白酶在免疫学上是相同的。

Starkey P M, Barrett A J

Biochem J. 1976 May 1;155(2):265-71. doi: 10.1042/bj1550265.

The elastase of human spleen was shown to exhibit endopeptidase activity against azo-casein and elastin. 2. Activity against several synthetic substrates was detected, and benzyloxycarbonyl-L-alanine 2-naphthyl ester was found to be a good substrate for routine use. 3. The enzyme showed a broad pH optimum in the range of 8.2-9.2 against azo-casein and the synthetic substrate. 4. The effect of inhibitors on the spleen elastase showed it to be a serine proteinase with a specificity similar to that of porcine pancreatic elastase. 5. Specific antisera were raised against the enzyme, and it was shown to be immunologically identical with the lysosomal elastase of human neutrophil leucocytes.

已证明人脾脏弹性蛋白酶对偶氮酪蛋白和弹性蛋白具有内肽酶活性。2. 检测到其对几种合成底物的活性，并且发现苄氧羰基-L-丙氨酸2-萘酯是常规使用的良好底物。3. 该酶在8.2 - 9.2范围内对偶氮酪蛋白和合成底物表现出较宽的pH最适值。4. 抑制剂对脾脏弹性蛋白酶的作用表明它是一种丝氨酸蛋白酶，其特异性与猪胰弹性蛋白酶相似。5. 制备了针对该酶的特异性抗血清，并且证明它与人类嗜中性白细胞的溶酶体弹性蛋白酶在免疫学上是相同的。