紫外线照射的猴肾细胞中的DNA连接酶活性。
DNA ligase activity in UV-irradiated monkey kidney cells.
作者信息
Mezzina M, Nocentini S
出版信息
Nucleic Acids Res. 1978 Nov;5(11):4317-28. doi: 10.1093/nar/5.11.4317.
Abstract
The DNA ligase activity of monkey kidney CV-1 cells has been measured at different stages of culture growth and after different time intervals following ultraviolet irradiation. Results indicate that: - The level of enzyme activity is about twice higher in non synchronous, rapidly dividing cells than in confluent cultures. - UV-irradiation of cells induces a "de novo" synthesis of DNA ligase. - This induction is dose dependent in its extent and kinetics, and may lead to a DNA ligase level in UV-irradiated stationary cultures of the same order as observed in unirradiated exponentially growing cells. - This induction seems to be independent of semiconservative DNA synthesis since it is not affected by fluorodeoxyuridine.
摘要
已在猴肾CV - 1细胞培养生长的不同阶段以及紫外线照射后的不同时间间隔测量了其DNA连接酶活性。结果表明：- 非同步快速分裂细胞中的酶活性水平比汇合培养细胞中的约高两倍。- 细胞的紫外线照射诱导DNA连接酶的“从头”合成。- 这种诱导在程度和动力学上是剂量依赖性的，并且可能导致紫外线照射的静止培养物中的DNA连接酶水平与未照射的指数生长细胞中观察到的水平相当。- 这种诱导似乎与半保留DNA合成无关，因为它不受氟脱氧尿苷的影响。
相似文献
1
DNA ligase activity in UV-irradiated monkey kidney cells.紫外线照射的猴肾细胞中的DNA连接酶活性。
Nucleic Acids Res. 1978 Nov;5(11):4317-28. doi: 10.1093/nar/5.11.4317.
2
DNA ligase activity in carcinogen-treated human fibroblasts.致癌物处理过的人成纤维细胞中的DNA连接酶活性
Biochimie. 1982 Aug-Sep;64(8-9):743-8. doi: 10.1016/s0300-9084(82)80122-3.
3
4
[Ligase activity of lig- bacteria infected with mu bacteriophage at non-permissive temperatures].[在非允许温度下感染了μ噬菌体的连接酶缺陷细菌的连接酶活性]
C R Seances Acad Sci D. 1979 Jan 15;288(2):259-62.
5
[Spontaneous DNA damage and DNA polynucleotide ligase activity in thymocyte populations differing in radiosensitivity].
Radiobiologiia. 1988 Jul-Aug;28(4):441-7.
6
Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents.响应DNA损伤剂，酵母DNA连接酶基因在指数生长期和稳定期培养物中的诱导表达。
Curr Genet. 1986;11(2):107-12. doi: 10.1007/BF00378201.
7
Induction of the SOS system by DNA ligase-deficient growth of Escherichia coli.大肠杆菌DNA连接酶缺陷型生长诱导SOS系统
J Gen Microbiol. 1982 Mar;128(3):613-21. doi: 10.1099/00221287-128-3-613.
8
Increased activity of polynucleotide ligase in 5-iodo-2'-deoxyuridine and mitomycin C-pretreated simian virus 40 (SV40)-infected monkey kidney cells.在经5-碘-2'-脱氧尿苷和丝裂霉素C预处理的感染猿猴病毒40（SV40）的猴肾细胞中，多核苷酸连接酶的活性增加。
Nucleic Acids Res. 1982 Aug 25;10(16):5073-84. doi: 10.1093/nar/10.16.5073.
9
UV-irradiated SV40 minichromosomes as substrates for DNA repair endonucleases.
Biochem Biophys Res Commun. 1983 May 31;113(1):309-16. doi: 10.1016/0006-291x(83)90467-9.
10
引用本文的文献
1
Heterogeneity of mammalian DNA ligase detected on activity and DNA sequencing gels.在活性凝胶和DNA测序凝胶上检测到的哺乳动物DNA连接酶的异质性。
Nucleic Acids Res. 1984 Jun 25;12(12):5109-22. doi: 10.1093/nar/12.12.5109.
2
Increased activity of polynucleotide ligase in 5-iodo-2'-deoxyuridine and mitomycin C-pretreated simian virus 40 (SV40)-infected monkey kidney cells.在经5-碘-2'-脱氧尿苷和丝裂霉素C预处理的感染猿猴病毒40（SV40）的猴肾细胞中，多核苷酸连接酶的活性增加。
Nucleic Acids Res. 1982 Aug 25;10(16):5073-84. doi: 10.1093/nar/10.16.5073.
3
Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents.响应DNA损伤剂，酵母DNA连接酶基因在指数生长期和稳定期培养物中的诱导表达。
Curr Genet. 1986;11(2):107-12. doi: 10.1007/BF00378201.
4
DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients.来自正常供体和布卢姆综合征患者的人类细胞系中的DNA连接酶活性。
Nucleic Acids Res. 1989 Apr 25;17(8):3091-106. doi: 10.1093/nar/17.8.3091.
5
A constitutive damage-specific DNA-binding protein is synthesized at higher levels in UV-irradiated primate cells.一种组成型损伤特异性DNA结合蛋白在紫外线照射的灵长类细胞中合成水平更高。
Mol Cell Biol. 1990 May;10(5):2041-8. doi: 10.1128/mcb.10.5.2041-2048.1990.
6
Benzamide potentiation of the cytotoxicity of bifunctional galactitol [correction of galacticol] in resistant P388 leukemia correlates with inhibition of DNA ligase II.苯甲酰胺增强双功能半乳糖醇（纠正：半乳糖醇）对耐药P388白血病细胞的细胞毒性作用与DNA连接酶II的抑制相关。
Cancer Chemother Pharmacol. 1992;30(4):325-9. doi: 10.1007/BF00686304.
7
Structure of the human DNA ligase I gene.人类DNA连接酶I基因的结构。
Nucleic Acids Res. 1992 Aug 11;20(15):3845-50. doi: 10.1093/nar/20.15.3845.
8
DNA polymerase alpha, beta and gamma activities in ultraviolet irradiated CV-1 monkey cells.紫外线照射的CV-1猴细胞中的DNA聚合酶α、β和γ活性
Nucleic Acids Res. 1979 Apr;6(4):1591-605. doi: 10.1093/nar/6.4.1591.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
CYCLOHEXIMIDE: ASPECTS OF INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS.环己酰亚胺：哺乳动物细胞中蛋白质合成抑制的相关方面
Science. 1964 Dec 11;146(3650):1474-6. doi: 10.1126/science.146.3650.1474.
3
Studies on fluorinated pyrimidines. XIII. Inhibition of thymidylate synthetase.氟化嘧啶的研究。十三。胸苷酸合成酶的抑制作用。
J Biol Chem. 1961 Nov;236:3006-13.
4
The relation between the synthesis of deoxyribonucleic acid and the synthesis of protein in the multiplication of bacteriophage T2.噬菌体T2增殖过程中脱氧核糖核酸合成与蛋白质合成之间的关系。
Biochem J. 1955 Nov;61(3):473-83. doi: 10.1042/bj0610473.
5
Polynucleotide ligase from rat liver after partial hepatectomy.
Biochem Biophys Res Commun. 1971 Mar 19;42(6):1156-61. doi: 10.1016/0006-291x(71)90026-x.
6
Induction of polynucleotide ligase in human lymphocytes stimulated by phytohemoagglutinin.植物血凝素刺激的人淋巴细胞中多核苷酸连接酶的诱导。
Biochem Biophys Res Commun. 1972 Jun 9;47(5):1221-7. doi: 10.1016/0006-291x(72)90965-5.
7
Enzymic joining of polynucleotides. 3. The polydeoxyadenylate-polydeoxythymidylate homopolymer pair.多核苷酸的酶促连接。3. 聚脱氧腺苷酸-聚脱氧胸苷酸同聚物对。
J Mol Biol. 1968 Sep 14;36(2):261-74. doi: 10.1016/0022-2836(68)90380-x.
8
Role of polynucleotide ligase in T4 DNA replication.多核苷酸连接酶在T4 DNA复制中的作用。
J Mol Biol. 1968 Aug 14;35(3):639-42. doi: 10.1016/s0022-2836(68)80020-8.
9
Polynucleotide ligase in mouse cells infected by polyoma virus.多瘤病毒感染的小鼠细胞中的多核苷酸连接酶。
Biochim Biophys Acta. 1972 May 29;269(3):385-96. doi: 10.1016/0005-2787(72)90126-8.
10
Increased activity of polynucleotide ligase from rat hepatoma induced by N-2-fluorenylacetamide.N-2-芴基乙酰胺诱导的大鼠肝癌中多核苷酸连接酶活性增加。
Cancer Res. 1972 May;32(5):886-8.
Zotero 插件