紫外线照射的CV-1猴细胞中的DNA聚合酶α、β和γ活性
DNA polymerase alpha, beta and gamma activities in ultraviolet irradiated CV-1 monkey cells.
作者信息
Wicker R, Scovassi A I, Nocentini S
出版信息
Nucleic Acids Res. 1979 Apr;6(4):1591-605. doi: 10.1093/nar/6.4.1591.
Abstract
To determine the possible role of DNA polymerase alpha, beta and gamma during the repair period following ultraviolet (lambda max : 254 nm) irradiation of monkey CV-1 cells, we measured the three enzymatic activities by using specific tests, either in crude extracts or after fractionation by sucrose gradient (5--20%) centrifugation at high salt concentration. When compared to the unirradiated control, we could not detect any significant variation in the levels of activity of DNA polymerases alpha, beta and gamma at any time (0, 12 to 48 h) after ultraviolet irradiation of the cells with doses ranging from 9 to 52.5 J.m-2.
摘要
为了确定DNA聚合酶α、β和γ在猴CV-1细胞经紫外线（λ最大值：254 nm）照射后的修复期可能发挥的作用，我们通过特定检测方法，在粗提物中或在高盐浓度下经蔗糖梯度（5%-20%）离心分级分离后，测定了这三种酶的活性。在用9至52.5 J.m-2的剂量对细胞进行紫外线照射后的任何时间点（0、12至48小时），与未照射的对照相比，我们均未检测到DNA聚合酶α、β和γ的活性水平有任何显著变化。
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