Andrezik J A, Chan-Palay V, Palay S L
Anat Embryol (Berl). 1981;161(4):373-90. doi: 10.1007/BF00316049.
Injections of horseradish peroxidase (HRP) were placed in the middle or caudal portion of the nucleus paragigantocellularis lateralis (PGCL) and 24 h later the entire spinal cord and brain were processed and examined for labeled neurons. Spinal afferents arise from all levels of the cord. Rexed's lamination scheme was adapted to the spinal cord of the rat and labelled neurons were localized to laminae IV, V, VII, VIII and X mainly on the side contralateral to the injection. At cervical levels, labeled neurons were consistently found bilaterally. The medial reticular nuclei of the medulla and pons contained HRP-labelled perikarya, which were concentrated most heavily in the nuclei reticularis medullae oblongatae ventralis, gigantocellularis, and pontis caudalis predominantly ipsilateral to the injection. The medial vestibular nucleus was consistently labeled. HRP-labeled perikarya were found bilaterally within the commissural portion and in the medial part of the nucleus of the solitary tract on the side of the injection. The rostral portion of the PGCL receives afferents from some secondary auditory nuclei: the ipsilateral inferior colliculus and the posterior ventral cochlear nucleus bilaterally. Thus, the rostral PGCL may be involved in auditory feedback loops. The caudal raphe nuclei are a major source of afferents to the caudal PGCL. The lateral hypothalamic area, paraventricular nucleus, and zona incerta also contain labeled neurons when injections are centered in the caudal portion of the nucleus.
将辣根过氧化物酶（HRP）注射到外侧巨细胞旁核（PGCL）的中部或尾部，24小时后，对整个脊髓和脑进行处理并检查标记神经元。脊髓传入纤维来自脊髓的各个节段。将 Rexed 分层模式应用于大鼠脊髓，标记神经元主要定位在注射对侧的IV、V、VII、VIII和X层。在颈髓节段，双侧均能持续发现标记神经元。延髓和脑桥的内侧网状核含有HRP标记的核周体，主要集中在注射同侧的延髓腹侧网状核、巨细胞网状核和脑桥尾侧网状核。内侧前庭核持续被标记。在注射侧，在孤束核的连合部和内侧部双侧发现HRP标记的核周体。PGCL的头端部分接受来自一些二级听觉核的传入纤维：同侧下丘和双侧蜗神经后腹核。因此，PGCL头端可能参与听觉反馈回路。尾部中缝核是PGCL尾端主要的传入纤维来源。当注射集中在该核的尾部时，下丘脑外侧区、室旁核和未定带也含有标记神经元。
