Spoor R P, Walerstein S J, Achtellik D M, Levine R L, Schiff M A
Physiol Chem Phys. 1978;10(1):73-8.
Quiescent rabbit left atria were exposed to ouabain for 200 min at various temperatures ranging from 27 degrees to 35 degrees C. Effects produced by the drug were noted when stimulation of the atria was resumed. At or below 31 degrees C, little effect on stimulation was seen and approximately 80 min were required to reach maximum effect of the drug. At and above 32 degrees C, effects could be seen immediately upon stimulation and only 45 min were required to reach maximum effect. In the range from 31 degrees to 32 degrees C, an increment of as little as 0.25 degrees altered both the time to reach maximum effect and the maximum force obtained. Experiments with tritiated ouabain showed no difference in uptake of the drug by atria, whether beating or quiescent, at 30 degrees C or 35 degrees C. Possible mechanisms are discussed.
将静态的兔左心房在27摄氏度至35摄氏度的不同温度下暴露于哇巴因200分钟。当恢复对心房的刺激时，记录药物产生的效应。在31摄氏度及以下，对刺激几乎没有影响，达到药物最大效应大约需要80分钟。在32摄氏度及以上，刺激后立即可见效应，仅需45分钟即可达到最大效应。在31摄氏度至32摄氏度范围内，温度仅升高0.25摄氏度就会改变达到最大效应的时间和所获得的最大力量。用氚标记的哇巴因进行的实验表明，在30摄氏度或35摄氏度时，无论心房是跳动还是静止，药物的摄取没有差异。文中讨论了可能的机制。
