雌二醇对大鼠下丘脑可溶性蛋白质合成的刺激作用。

Stimulation by oestradiol of soluble-protein synthesis in rat hypothalamus.

作者信息

Vértes M, Környei J, Nagy L, Vértes Z, Kovács S

出版信息

Mol Cell Endocrinol. 1981 Jun;22(3):329-38. doi: 10.1016/0303-7207(81)90041-1.

Abstract

The effect of oestradiol treatment on protein synthesis in the cytosol fraction (10(5) g supernatant) from ovariectomized mature and developing female rat hypothalami were studied. Results obtained on adults by double-label technique and SDS polyacrylamide gel or cellogel electrophoresis show that, as in the uterus, oestradiol-induced protein synthesis (IP) occurred in the cytosol fraction of the hypothalamus. The molecular weight of IP was about 40 000 dalton. During postnatal development, IP was also observed in cytosol fractions from the hypothalami of female rats 14, 21 and 28 days old. No clear-cut effect of oestradiol was found in the 7-day-old animals.

摘要

研究了雌二醇处理对去卵巢的成年和发育中雌性大鼠下丘脑胞质部分（10⁵g 上清液）蛋白质合成的影响。通过双标记技术以及 SDS 聚丙烯酰胺凝胶或细胞凝胶电泳对成年大鼠获得的结果表明，与子宫中一样，雌二醇诱导的蛋白质合成（IP）发生在下丘脑的胞质部分。IP 的分子量约为 40000 道尔顿。在出生后的发育过程中，在 14、21 和 28 日龄雌性大鼠下丘脑的胞质部分也观察到了 IP。在 7 日龄动物中未发现雌二醇有明显作用。