Környei J, Székely J A, Vértes M
J Steroid Biochem. 1983 May;18(5):535-40. doi: 10.1016/0022-4731(83)90128-0.
The effect of an in vitro application of oestradiol-17-beta on the soluble protein synthesis in human endometrium during the menstrual cycle was investigated. Results obtained by double-label technique and SDS-polyacrylamide gel or Cellogel electrophoresis show that synthesis of soluble proteins in human endometrium is affected by oestradiol in both follicular and luteal phases of the cycle. One of the proteins obtained after oestradiol treatment seems to be similar to IP of rat uterus as judged from its molecular weight (41,000 daltons) and electrophoretic mobility (RF:1.18-1.24 relative to BSA on Cellogel). The magnitude of this protein synthesis correlates significantly with cytoplasmic oestradiol receptor concentration in endometrium throughout the cycle. The highest induction of IP like protein(s) synthesis at follicular phase and a decreased induction during the luteal phase was detected. No quantitative relationship between the changes of progesterone receptor concentration and IP like protein synthesis was found.
研究了体外应用17-β-雌二醇对月经周期中人子宫内膜可溶性蛋白质合成的影响。通过双标记技术以及SDS-聚丙烯酰胺凝胶或Cellogel电泳获得的结果表明，在月经周期的卵泡期和黄体期，人子宫内膜中可溶性蛋白质的合成均受雌二醇影响。从其分子量（41,000道尔顿）和电泳迁移率（在Cellogel上相对于牛血清白蛋白的相对迁移率RF：1.18 - 1.24）判断，雌二醇处理后获得的一种蛋白质似乎与大鼠子宫的IP相似。在整个周期中，这种蛋白质合成的程度与子宫内膜中的细胞质雌二醇受体浓度显著相关。在卵泡期检测到IP样蛋白合成的诱导最高，而在黄体期诱导降低。未发现孕酮受体浓度变化与IP样蛋白合成之间的定量关系。
