Fromtling R A, Blackstock R, Hall N K, Bulmer G S
Infect Immun. 1979 May;24(2):449-53. doi: 10.1128/iai.24.2.449-453.1979.
A murine model was developed to study the cell-mediated immune response of mice immunized with one of two live, avirulent forms of Cryptococcus neoformans: a nonencapsulated mutant and a thinly encapsulated pseudohyphal variant. A lymphocyte transformation assay was used to evaluate the cellular response of control and sensitized spleen cells after in vitro incubation with three merthiolate-killed whole-cell antigens of C. neoformans. An antigen-to-spleen cell ratio of 10:1 and 5 days of incubation of antigen-spleen cell mixtures were established as optimal conditions for maximum lymphocyte transformation. Maximum responses occurred from 2 to 3 weeks after the last of eight weekly intraperitoneal inoculations of C. neoformans. This assay provided an accurate, reproducible method of studying cell-mediated immunity to C. neoformans, and applications to the study of cryptococcal pathogenesis are proposed.
建立了一种小鼠模型，用于研究用新型隐球菌的两种活的无毒形式之一免疫的小鼠的细胞介导免疫反应：一种非包膜突变体和一种薄包膜假菌丝变体。淋巴细胞转化试验用于评估对照和致敏脾细胞在体外用三种硫柳汞灭活的新型隐球菌全细胞抗原孵育后的细胞反应。确定抗原与脾细胞的比例为10:1以及抗原-脾细胞混合物孵育5天是实现最大淋巴细胞转化的最佳条件。在每周一次腹腔注射新型隐球菌共八次的最后一次注射后2至3周出现最大反应。该试验提供了一种准确、可重复的研究针对新型隐球菌的细胞介导免疫的方法，并提出了其在隐球菌发病机制研究中的应用。
