Snippe H, De Reuver M J, Strickland F, Willers J M, Hunter R L
Int Arch Allergy Appl Immunol. 1981;65(4):390-8. doi: 10.1159/000232780.
The adjuvant activities of four chemically similar, but physicochemically different nonionic surface-active agents called pluronic polyols F 68, L 31, L 101 and L 121 were studied. These four agents were tested in mice using an experimental model developed for studying the adjuvant activity of the cationic surface-active agent dimethyl dioctadecyl ammonium bromide (DDA). L 121 and DDA enhanced the primary antibody response to sheep red blood cells (SRBC) while F 68, L 31 and L 101 suppressed this response. The secondary humoral response to SRBC was enhanced by the polyol L 121 while the secondary response to dinitrophenylated bovine serum albumin (DNP22-BSA) was enhanced by both L 121 and L 101. DDA and the polyol L 101 were very effective adjuvants for induction of delayed-type hypersensitivity (DTH) to SRBC and DNP22-BSA after intracutaneous immunization of mice with a mixture of antigen and adjuvant. Since the four pluronic polyols were composed of identical chemical constituents, we proposed that difference in their activities as adjuvants were due to variation in their physicochemical properties. A correlation was found between a physicochemical parameter, the hydrophilelipophile balance (HLB), and the adjuvant activities of the pluronic polyols and several other types of nonionic surface-active agents. The agents which were strong adjuvants all had HLB values within a narrow range which classified them as spreading agents.
研究了四种化学结构相似但物理化学性质不同的非离子表面活性剂，即普朗尼克多元醇F 68、L 31、L 101和L 121的佐剂活性。使用为研究阳离子表面活性剂二甲基二十八烷基溴化铵（DDA）的佐剂活性而开发的实验模型，在小鼠中对这四种试剂进行了测试。L 121和DDA增强了对绵羊红细胞（SRBC）的初次抗体反应，而F 68、L 31和L 101则抑制了这种反应。多元醇L 121增强了对SRBC的二次体液反应，而L 121和L 101均增强了对二硝基苯基化牛血清白蛋白（DNP22-BSA）的二次反应。在用抗原和佐剂混合物对小鼠进行皮内免疫后，DDA和多元醇L 101是诱导对SRBC和DNP22-BSA的迟发型超敏反应（DTH）的非常有效的佐剂。由于这四种普朗尼克多元醇由相同的化学成分组成，我们提出它们作为佐剂的活性差异是由于其物理化学性质的变化。发现物理化学参数亲水亲油平衡（HLB）与普朗尼克多元醇和其他几种类型的非离子表面活性剂的佐剂活性之间存在相关性。强佐剂的试剂的HLB值都在一个狭窄的范围内，这将它们归类为铺展剂。
