Davis W L, Jones R G, Hagler H K
J Histochem Cytochem. 1981 May;29(5):601-8. doi: 10.1177/29.5.7252127.
Transmission electron microscopy, electron microprobe analysis, high temperature microincineration, and electron microscopic histochemical procedures were used to study the electron-dense deposits characteristic of the macular aspect of aged human eyes. These inorganic deposits were rich in calcium and phosphorus and selectively removed by flotation on formic acid. The amorphous decalcified masses showed a significant sulfur peak and were readily stained with acidic phosphotungstic acid. The latter observations are indicative of the presence of organic matrical proteoglycan. Such data may be a further indication that proteoglycans are retained at sites of calcification.
运用透射电子显微镜、电子微探针分析、高温微量灰化以及电子显微镜组织化学方法，对老年人类眼睛黄斑区域特有的电子致密沉积物进行了研究。这些无机沉积物富含钙和磷，并通过在甲酸上浮选法被选择性去除。无定形脱钙团块显示出显著的硫峰，且很容易被酸性磷钨酸染色。后一观察结果表明存在有机基质蛋白聚糖。这些数据可能进一步表明蛋白聚糖保留在钙化部位。
