克隆人类胎儿γ珠蛋白和小鼠α型珠蛋白DNA：特征分析与部分测序
Cloning human fetal gamma globin and mouse alpha-type globin DNA: characterization and partial sequencing.
作者信息
Smithies O, Blechl A E, Denniston-Thompson K, Newell N, Richards J E, Slightom J L, Tucker P W, Blattner F R
出版信息
Science. 1978 Dec 22;202(4374):1284-9. doi: 10.1126/science.725604.
Two globin-related clones isolated from collections of bacteriophages containing unfractionated Eco RI fragments of human and mouse DNA were characterized. Charon3AHs51.1Hbgamma includes 2.7 kilobase pairs of human DNA containing a large part of a fetal gamma globin chain structural gene; Charon 3AMm30.5 includes 4.7 kilobase pairs of mouse DNA related to alpha globin. The human fetal gamma globin gene has within its coding region two intervening sequences of noncoding DNA, IVS 1 and IVS 2, of approximately 1-0 and 900 base pairs. Sequence IVS 1 is located at the position of one of the two intervening sequences occurring in adult globin genes; IVS 2 is located at the position of the other.
对从包含人类和小鼠DNA未分级Eco RI片段的噬菌体文库中分离出的两个与珠蛋白相关的克隆进行了表征。Charon3AHs51.1Hbgamma包含2.7千碱基对的人类DNA，其中含有胎儿γ珠蛋白链结构基因的一大部分；Charon 3AMm30.5包含4.7千碱基对与α珠蛋白相关的小鼠DNA。人类胎儿γ珠蛋白基因在其编码区内有两个非编码DNA间隔序列，IVS 1和IVS 2，分别约为100和900个碱基对。序列IVS 1位于成人珠蛋白基因中出现的两个间隔序列之一的位置；IVS 2位于另一个的位置。
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