从猪脊髓中分离并部分鉴定两种生物活性肽

Isolation and partial characterization of two biologically active peptides from porcine spinal cord.

作者信息

Xi G L, Hua J C, Chen R L, Zhang R B, Zou G, Gao Y S

出版信息

Sci Sin. 1981 Jul;24(7):1010-20.

Abstract

Two biologically active peptides designated SCP-1 and SCP-2 have been isolated and partially characterized from porcine spinal cord. The results of amino acid analysis, electrophoresis, isoelectric focusing and the chromatographic analysis of the dansylated derivatives indicate that these two peptides are homogeneous. The amino acid composition and apparent untriakontapeptide and SCP-2 is a pentacospeptide. The N-terminal amino acids of SCP-1 and SCP-2 are Arginine and Tryptophan respectively. SCP-1 can stimulate the contraction of isolated guinea pig ileum and SCP-2 induces hypotension in anesthetized rat. Both SCP-1 and SCP-2 show no morphine-like activity. The chemical composition and biological properties of SCP-1 and SCP-2 distinguish them from any known peptide.

摘要

从猪脊髓中分离出了两种具有生物活性的肽，分别命名为SCP - 1和SCP - 2，并对其进行了部分特性鉴定。氨基酸分析、电泳、等电聚焦以及丹磺酰化衍生物的色谱分析结果表明，这两种肽是均一的。氨基酸组成以及表观上SCP - 1是一个三十三联肽，SCP - 2是一个二十五肽。SCP - 1和SCP - 2的N端氨基酸分别是精氨酸和色氨酸。SCP - 1能刺激豚鼠离体回肠收缩，SCP - 2能使麻醉大鼠血压降低。SCP - 1和SCP - 2均无吗啡样活性。SCP - 1和SCP - 2的化学组成和生物学特性使其有别于任何已知的肽。