Machiedo G W, Ghuman S, Rush B F, Kraven T, Dikdan G
Surgery. 1981 Aug;90(2):328-35.
The ability of exogenous ATP-MgCl2 to reverse the inhibition of ATP-dependent intracellular reactions by hemorrhagic shock was studied. Levels of ornithine in the postperfusion fluid were lower in animals receiving ATP-MgCl2 than in placebo-treated control animals (338.6 +/- 167.0 versus 692.1 +/- 67.2 mumol). Arginine levels were higher (399.1 +/- 130.1 versus 34.3 +/- 59.1 mumol) in ATP-MgCl2-treated animals. Ability of in vitro ATP to enter the cell and inhibit lactate formation (Crabtree effect) was significantly less in those animals receiving in vivo ATP-MgCl2 (81.4 +/- 11.1% versus 57.7 +/- 10.1%). Glutamate levels were not decreased by shock but were significantly increased by treatment with ATP-MgCl2 compared to placebo (190.5 +/- 48.8 versus 122.6 +/- 36.3 mumol). These data indicate that exogenously administered ATP-MgCl2 can reverse the inhibition of ornithine metabolism and the changes in lactate inhibition seen in hemorrhagic shock. These are both intracellular ATP-dependent reactions.
研究了外源性ATP-MgCl₂逆转失血性休克对ATP依赖性细胞内反应抑制作用的能力。接受ATP-MgCl₂的动物灌注后液体中的鸟氨酸水平低于接受安慰剂治疗的对照动物（338.6±167.0对692.1±67.2μmol）。接受ATP-MgCl₂治疗的动物精氨酸水平较高（399.1±130.1对34.3±59.1μmol）。接受体内ATP-MgCl₂的动物中，体外ATP进入细胞并抑制乳酸生成（Crabtree效应）的能力显著降低（81.4±11.1%对57.7±10.1%）。休克并未降低谷氨酸水平，但与安慰剂相比，ATP-MgCl₂治疗显著增加了谷氨酸水平（190.5±48.8对122.6±36.3μmol）。这些数据表明，外源性给予ATP-MgCl₂可逆转失血性休克中鸟氨酸代谢的抑制和乳酸抑制的变化。这两者都是细胞内ATP依赖性反应。
