Holme R, Oftebro R, Hovig T
Thromb Haemost. 1978 Aug 31;40(1):89-102.
The effects of washed human cultured cells (tumour cells and Chang liver cells) on human blood platelets in heparinized plasma were studied. Platelet aggregation was induced by suspensions of the tumour cells. Ultrastructural examination showed that the platelets, especially in the central regions of the aggregates, were tightly packed and the alpha-granules were mostly present. In the periphery of the aggregates the platelets appeared swollen and devoid of organelles, and fibrin strands were seen. The platelet aggregation was not completely abolished by incubation with apyrase. The washing fluids from the tumour cells also induced platelet aggregation, but the aggregation could be abolished by incubation with apyrase. When three different lines of the Chang cells were used, suspensions of two lines of these cells induced platelet aggregation, but the third line did not. Presence of ADP could be demonstrated in the washing fluids from the cultured cells, except for the one line of Chang cells which did not induce platelet aggregation. The experiments indicated that the platelet aggregation induced by the various types of cells was mediated via ADP, but with a possible additional effect of coagulation activity.
研究了经洗涤的人培养细胞（肿瘤细胞和张氏肝细胞）对肝素化血浆中人体血小板的影响。肿瘤细胞悬液可诱导血小板聚集。超微结构检查显示，血小板，尤其是在聚集体的中心区域，紧密堆积，且大多存在α颗粒。在聚集体的周边，血小板似乎肿胀且缺乏细胞器，可见纤维蛋白丝。用腺苷三磷酸双磷酸酶孵育不能完全消除血小板聚集。肿瘤细胞的洗涤液也可诱导血小板聚集，但用腺苷三磷酸双磷酸酶孵育可消除这种聚集。当使用三种不同株系的张氏细胞时，其中两株系的细胞悬液可诱导血小板聚集，但第三株系则不能。除了不诱导血小板聚集的那一株系张氏细胞外，在培养细胞的洗涤液中可检测到二磷酸腺苷的存在。实验表明，各种类型细胞诱导的血小板聚集是通过二磷酸腺苷介导的，但可能还有凝血活性的额外作用。
